81349

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt

powder

• Synonim(y): Poly(dI-dC) sodium salt
• Numer CAS: 118578-37-3
• UNSPSC Code: 12352106
• NACRES: NA.52
• MDL number: MFCD00147947
• Form: powder
• Solubility: water: 1 mL/vial, clear, colorless
• Storage temp.: −20°C

Właściwości

• Nazwa produktu: Poly(2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt, free from low molecular weight nucleotides, lyophilized
• grade: Molecular Biology
• Quality Segment: 100
• form: powder
• quality: lyophilized
• mol wt: M_r ~2.5 × 10⁶ (broad molecular weight distribution), M_r ~2500000
• packaging: ampule of ≥10 units A₂₆₀
• solubility: water: 1 mL/vial, clear, colorless
• storage temp.: −20°C

Opis

General description

Poly (2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt is an alternating copolymer that acts as a substrate for DNA methyltransferases.

Application

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt has been used in electrophoretic mobility shift assay (EMSA) of the transcription factor AP-2 Alpha (TFAP2A) and in southwestern blotting of human embryonic kidney cells (HEK293) nuclear extract.

Suitable for

• non-specific blocking agent in EMSA
• evaluation of DNA methyltransferases
• DNA-small molecule or drug interaction studies

Biochem/physiol Actions

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid), poly(dI-dC) is a synthetic DNA substrate. At high salt, Poly(dI-dC) exists in left handed helical conformation and reverts to right-handed form upon decreasing salt. It is used as a non-specific competitor in electrophoretic mobility shift assay (EMSA).

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• Co się dzieje?: WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• PPE (środki ochrony indywidualnej): Eyeshields, Gloves, type N95 (US)