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83264

Sigma-Aldrich

HEPES buffer solution

1 M in H2O

Synonym(s):
HEPES solution, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
CAS Number:
Numer MDL:
eCl@ss:
32129211
Identyfikator substancji w PubChem:
NACRES:
NA.25

forma

liquid

Poziom jakości

100

stężenie

1 M in H2O
238 g/L HEPES in H2O

zanieczyszczenia

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH

5.5±0.5

useful pH range

6.8-8.2

gęstość

1.07 g/mL at 20 °C

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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Opis ogólny

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Zastosowanie

HEPES has been used:
  • As a component of SAPI medium used for the culturing of S. Typhymurium inoculum
  • To prepare differentiation medium for PC12 cells. It is also a component of culture medium RPMI
  • As a component of dissection buffer for culturing rat cortical neurons
  • Along with MgCl2, KCl and Spermine in hypotonic buffer in mGRASP method to detect mammalian synapses
  • As a component of Danieu′s aquarium water used in agarose gel preparation

Kod klasy składowania

12 - Non Combustible Liquids

WGK

WGK 1

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificate of Analysis

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Certificate of Origin

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