This product has not been validated for immunohistochemistry (IHC). However, numerous citations exist using this product for IHC.
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G1544
Anti-GFP, N-terminal antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonim(y):
Anti-Green Fluorescent Protein
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
100 μg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 4320,00 zł |
Informacje o tej pozycji
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP, WB
Citations:
300
4320,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócbiological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 1.0-2.5 μg using GFP fusion protein from transfected mammalian cell lysates, western blot: 0.25-0.5 μg/mL using 3-6 ng of purified GFP fusion proteins expressed in mammalian cell extracts
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Green fluorescent protein (GFP) chromophore contains hexapeptide fragment with Phe64-Ser-Tyr-Gly-Val-Gln69 aminoacid sequence. GFP structure contains a 11-stranded β-barrel and a central helix and corresponds to a molecular weight of 26 kDa.
Immunogen
synthetic peptide corresponding amino acids 3-17 of GFP.
Application
Anti-GFP, N-terminal antibody produced in rabbit has been used:
- in immunoblotting
- in the histochemistry and immunohistochemistry
- in the immunoprecipitation
Biochem/physiol Actions
Green fluorescent protein (GFP) has photochemical and diagnostic applications. It is also used widely in protein dynamics studies. The polyclonal anti-GFP, N-terminal rabbit antibody reacts specifically with GFP fusion proteins.
Specific for GFP fusion proteins.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
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Ta pozycja | |||
|---|---|---|---|
| description ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution | description clone GSN24, purified from hybridoma cell culture | description clone GSN149, purified from hybridoma cell culture | description clone GFP-20, ascites fluid |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| antibody form affinity isolated antibody | antibody form purified immunoglobulin | antibody form purified from hybridoma cell culture | antibody form ascites fluid |
| biological source rabbit | biological source mouse | biological source mouse | biological source mouse |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| technique(s) immunoprecipitation (IP): 1.0-2.5 μg using GFP fusion protein from transfected mammalian cell lysates, western blot: 0.25-0.5 μg/mL using 3-6 ng of purified GFP fusion proteins expressed in mammalian cell extracts | technique(s) western blot: 1-2 μg/mL using GFP fusion proteins expressed in extracts of transfected cells | technique(s) indirect ELISA: suitable, western blot: 1-2 μg/mL using extracts of cells expressing GFP fusion proteins | technique(s) western blot: 1:2,000 using purified recombinant GFP preparation, dot blot: suitable, indirect ELISA: suitable |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
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Klasa składowania
10 - Combustible liquids
WGK
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
PPE (środki ochrony indywidualnej)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Can this antibody be used in IHC?
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