Merck
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11814460001

Roche

Anti-GFP

from mouse IgG1κ (clones 7.1 and 13.1)

Synonym(s):
anti-green fluorescent protein

pochodzenie biologiczne

mouse

Poziom jakości

100

białko sprzężone

unconjugated

forma przeciwciała

purified immunoglobulin

antibody product type

primary antibodies

klon

13.1, monoclonal
7.1, monoclonal

próba

>90% (HPLC)

forma

lyophilized

opakowanie

pkg of 200 μg

manufacturer/tradename

Roche

izotyp

IgG1κ

transport

wet ice

temp. przechowywania

2-8°C

Opis ogólny

Green Fluorescent Protein (GFP) is a spontaneously fluorescent 27kDa protein originally isolated from the jellyfish Aequorea victoria. The molecular cloning of the GFP gene and its subsequent expression in heterologous systems has established GFP as a valuable reporter molecule for in vivo visualization of gene expression events in a wide variety of cell types and organisms. Since, GFP requires no additional substrates or cofactors, GFP′s green fluorescence can be easily detected using blue or UV light after expression in either prokaryotic or eukaryotic cells. In addition, several mutant forms of GFP with unique spectral properties (e.g., enhanced fluorescence signal and shifts in excitation and emission spectra) have been reported.
Mixture of two high-affinity monoclonal antibodies selected for their performance in detection of GFP and GFP fusion proteins.

Specyficzność

Subtype: Both clones are Mouse IgG1κ

Zastosowanie

Monoclonal antibody for detection of both wild-type and mutant forms of GFP or GFP fusions using:
  • Immunoprecipitation
  • Western blots
  • Immunostaining

Cechy i korzyści

Contents
Mixture of two monoclonal antibodies, supplied as a white lyophilizatecontaining 200μg of total Anti-GFP IgG.
Anti-GFP is a mixture of two clones (7.1 and 13.1).

Jakość

Anti-GFP is tested for functionality and purity relative to a reference standard to confirm the quality of each new reagent preparation.
Purity: Both Anti-GFP mouse monoclonal antibodies (Clones 7.1 and 13.1) are >95% pure as determined by SDS-PAGE and ion-exchange HPLC analyses.

Uwaga dotycząca przygotowania

Working concentration: Working concentration of antibody depends on application and substrate.
The following concentrations should be taken as a guideline:
  • Western blot: 1:1000 dilution
  • Immunoprecipitation: 2 to 10 μg

Storage conditions (working solution): -15 to -25 °C

Rekonstytucja

Add 500 μl double distilled water to a final concentration of 0.4 mg/ml.
Rehydrate on ice for 30 minutes.

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

Informacje prawne

This product is sold under license from Columbia University. Rights to use this product are limited to research use only. No other rights are conveyed. Inquiry into the availability of a license to broader rights or the use of this product for commercial purposes should be directed to Columbia Innovation Enterprise, Columbia University, Engineering Terrace - Suite 363, new York, New York 10027.

Kod klasy składowania

13 - Non Combustible Solids

WGK

WGK 1

Temperatura zapłonu °F

does not flash

Temperatura zapłonu °C

does not flash

Certificate of Analysis

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Certificate of Origin

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