Yes. The columns fit on all standard vacuum manifolds.
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NA0400
GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit
sufficient for 10 preparations
Cechy związane ze zrównoważonym rozwojem
Synonim(y):
Gen Elute, GenElute Endotoxin-Free Plasmid Kit
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
1 kit | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1780,00 zł |
Informacje o tej pozycji
NACRES:
NA.52
UNSPSC Code:
41105501
eCl@ss:
32160703
1780,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócusage
sufficient for 10 preparations
Quality Segment
sustainability
Sustainability features
technique(s)
DNA extraction: suitable
test parameters
sample volume: 150 mL bacterial culture
storage temp.
15-25°C
General description
This product aligns with one or more of the 12 Principles of Green ChemistryThis product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
The GenElute HP Endotoxin-Free Plasmid Maxiprep Kit offers a simple and rapid method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. The kit uses a vacuum format with a filter column for the rapid clearing of the bacterial lysate and a silica column for capturing plasmid DNA. A proprietary solution binds plasmid DNA to the binding column while preventing endotoxins from adsorbing. The technology allows for the user to consistently achieve levels of endotoxin that are less than 0.1 endotoxin units per μg of plasmid DNA.
High-quality, endotoxin-free DNA is ready for immediate use for the most demanding applications including transfection with endotoxin-sensitive cells.
High-quality, endotoxin-free DNA is ready for immediate use for the most demanding applications including transfection with endotoxin-sensitive cells.
Biochem/physiol Actions
An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis. The lysate is clarified by filtration followed by the addition of a binding solution that has been optimized for endotoxin-free plasmid preparations. The plasmid DNA is then captured on silica, while endotoxins are prevented from adsorbing to the membrane. Two wash steps remove contaminants. Finally, the bound DNA is eluted in endotoxin-free water. The recovered plasmid DNA is predominately in its supercoiled form. Genomic DNA and RNA are below detectable levels by ethidium bromide stained agarose gel electrophoresis.
Features and Benefits
- Up to 1.2 mg of high-copy plasmid DNA with endotoxin levels of ≤0.1 EU/μg
- Only 35 minutes from pelleted cells to purified plasmid
- Convenient vacuum format
Other Notes
For additional information, please see www.sigma-aldrich.com/genelutehp.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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Ta pozycja | |||
|---|---|---|---|
| description sufficient for 10 preparations | description sufficient for 15 purifications | description sufficient for 10 purifications | description sufficient for 25 preparations |
| technique(s) DNA extraction: suitable | technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) DNA extraction: suitable |
| usage sufficient for 10 preparations | usage sufficient for 15 purifications | usage sufficient for 10 purifications | usage sufficient for 25 preparations |
| test parameters sample volume: 150 mL bacterial culture | test parameters sample volume: 25-200 mL bacterial culture, : ~45 min kit run time | test parameters sample volume: 50 mL bacterial culture | test parameters sample volume: 150 mL bacterial culture |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C |
| greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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Słowo ostrzegawcze
Danger
Kody zagrożeń
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Narządy docelowe
Central nervous system
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
WGK
WGK 3
Klasa składowania
10 - Combustible liquids
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Do the binding columns fit on other manufacturers’ manifolds?
1 answer-
Helpful?
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How do the GenElute HP Plasmid Kits differ from other plasmid purification systems?
1 answer-
The GenElute HP Plasmid Kits are much faster than other commercially available kits. A filter syringe is used for preparing the cleared lysate, which replaces a lengthy centrifugation step. These kits also offer the option of using a vacuum or spin purification format rather than a gravity drip to bind, wash and elute the plasmid. In addition, samples are eluted in Tris buffer so there are no alcohol precipitations required.
Helpful?
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What is the expected yield of plasmid DNA that you can isolate from these kits?
1 answer-
Yields can reach as high as 350 mg for a midiprep and 1.2 mg for a maxiprep. As with any protocol, lower yields can be expected with low copy plasmids.
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How long does it take to isolate plasmid using the vacuum format?
1 answer-
A midiprep or maxiprep requires only 30 minutes to complete.
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Is plasmid DNA isolated with this kit suitable for use in transfection?
1 answer-
Yes. However, if you are currently required to use an endofree plasmid purification kit, we recommend using the GenElute HP Endotoxin-free Kit.
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Which format gives a better yield, vacuum or spin?
1 answer-
There is no difference in plasmid recovery when using either format.
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