A211100

DMT-2′O-Methyl-rA(bz) Phosphoramidite

• Synonim(y): DMT-2′-O-Me-rA(bz) amidite, N-benzoyl-5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-O-methyl-adenosine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite]
• Wzór empiryczny (zapis Hilla): C₄₈H₅₄N₇O₈P
• Numer CAS: 110782-31-5
• Masa cząsteczkowa: 887.96
• NACRES: NA.51
• PubChem Substance ID: 329770740
• UNSPSC Code: 41116105
• MDL number: MFCD00792622
• Assay: ≥99% (³¹P-NMR), ≥99.0% (reversed phase HPLC)
• Biological source: non-animal source (no BSE/TSE risk)
• Form: powder
• Storage temp.: -10 to -25°C

Właściwości

• biological source: non-animal source (no BSE/TSE risk)
• Quality Segment: 300
• product line: Proligo Reagents
• assay: ≥99% (³¹P-NMR), ≥99.0% (reversed phase HPLC)
• form: powder
• technique(s): oligo synthesis: suitable
• impurities: ≤0.1% single unspecified Impurity (reversed phase HPLC), ≤0.3% mA2 (reversed phase HPLC, Hydrolysate), ≤0.3% mA3 (reversed phase HPLC, DMT-rA(bz)me), ≤0.3 wt. % water content (Karl Fischer), ≤0.5% P(III) Impurities 100-169ppm (³¹P-NMR), ≤1.0% mA1 (reversed phase HPLC, DMT-rA(bz)me-DMT), ≤3 wt. % residual Solvent content
• color: white to off-white
• λ: conforms (UV/VIS Identity)
• suitability: conforms to structure for H-NMR, conforms to structure for LC-MS
• nucleoside profile: base: adenosine; base protecting group: benzoyl; 2' protecting group: methyl; 5' protecting group: DMT; deprotection: standard
• storage temp.: -10 to -25°C
• SMILES string: CO[C@@H]1[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(c2ccccc2)(c3ccc(OC)cc3)c4ccc(OC)cc4)O[C@H]1n5cnc6c(NC(=O)c7ccccc7)ncnc56
• InChI: 1S/C48H54N7O8P/c1-32(2)55(33(3)4)64(61-28-14-27-49)63-42-40(62-47(43(42)59-7)54-31-52-41-44(50-30-51-45(41)54)53-46(56)34-15-10-8-11-16-34)29-60-48(35-17-12-9-13-18-35,36-19-23-38(57-5)24-20-36)37-21-25-39(58-6)26-22-37/h8-13,15-26,30-33,40,42-43,47H,14,28-29H2,1-7H3,(H,50,51,53,56)/t40-,42-,43-,47-,64?/m1/s1
• InChI key: AZCGOTUYEPXHMJ-PSVHYZMASA-N

Opis

General description

DMT-2′O-Methyl-rA(bz) Phosphoramidite belongs to the class of 2′O-Methyl RNA Phosphoramidites. 2′O-Methyl RNA monomers are compatible with fast deprotection schemes that are based on the application of aliphatic amines, such as methylamine. 2′O-Methyl RNA is a nucleic acid analog that is characterized by the exceptional hybridization properties that it imparts with complementary DNA or RNA, as well as increased stability against enzymatic degradation compared to natural nucleic acids. 2′O-Methyl RNA monomers feature methoxy groups at the 2′-position. The methoxy groups are perfectly stable in all conditions employed in the assembly of oligonucleotides by automated phosphoramidite synthesis, and in all standard alkaline deprotection conditions.

Application

DMT-2′O-Methyl-rA(bz) Phosphoramidite is suitable for use in the preparation of oligonucleotide probes.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable