L-Ascorbic acid

powder, suitable for cell culture, γ-irradiated

Antiscorbutic factor, Vitamin C, L-Threoascorbic acid
Empirical Formula (Hill Notation):
Numer CAS:
Masa cząsteczkowa:
Beilstein/REAXYS Number:
Numer EC:
Numer MDL:
Identyfikator substancji w PubChem:

Poziom jakości


pochodzenie biologiczne

synthetic (organic)





masa cząsteczkowa

Mw 176.12 g/mol


cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable


white to off-white


1.0-2.5 (25 °C, 176 g/L in water)


190-194 °C (dec.)


H2O: 10 mg/mL

SMILES string




InChI key


Gene Information

human ... SLC23A2(9962)

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Opis ogólny

L-ascorbic acid is an unsaturated γ-lactone ring containing an enediol configuration (–COH=COH) attached with a carbonyl group.


For use in cell culture applications as an antioxidant and to regenerate tocopherol.
L-ascorbic acid has been used:
  • in an osteogenic supplement for the growth of cells
  • as a component in the differentiation media
  • in WS-MSCs (Werner syndrome-mesenchymal stem cells) to study rescuing properties of ascorbic acid on aging defects

Działania biochem./fizjol.

L-ascorbic acid mainly exhibits antioxidant properties. It protects plants from oxidative stress and mammals from diseases associated with oxidative stress. L-ascorbic acid mainly protects from hydroxyl radicals, superoxide and singlet oxygen. In addition, it also reduces the membrane-linked antioxidant α-tocopherol (oxidised form). L-Ascorbic acid enhances endothelium-dependent vasodilation in various disorders, including diabetes, coronary artery disease, hypertension and chronic heart failure.


13 - Non Combustible Solids

WGK Germany


Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)

Certyfikat analizy

Świadectwo pochodzenia

Xi Wang et al.
Archives of oral biology, 57(9), 1231-1240 (2012-03-30)
This study focused on the characterization of stem cells from human exfoliated deciduous teeth (SHED) in comparison with dental pulp stem cells (DPSCs) to certify SHED as a key element in tissue engineering. In the present study, SHED and DPSCs...
Plant L-ascorbic acid: chemistry, function, metabolism, bioavailability and effects of processing.
Davey MW
Journal of the Science of Food and Agriculture, 80, 825-860 (2000)
R Heller et al.
The Journal of biological chemistry, 274(12), 8254-8260 (1999-03-13)
Ascorbic acid has been shown to enhance impaired endothelium-dependent vasodilation in patients with atherosclerosis by a mechanism that is thought to involve protection of nitric oxide (NO) from inactivation by free oxygen radicals. The present study in human endothelial cells...
P L Conklin et al.
Plant physiology, 115(3), 1277-1285 (1997-12-09)
The biosynthesis of L-ascorbic acid (vitamin C) is not well understood in plants. The ozone-sensitive Arabidopsis thaliana mutant vitamin c-1 (vtc1; formerly known as soz1) is deficient in ascorbic acid, accumulating approximately 30% of wild-type levels. This deficiency could result...
Ying Li et al.
Protein & cell, 7(7), 478-488 (2016-06-09)
Werner syndrome (WS) is a premature aging disorder that mainly affects tissues derived from mesoderm. We have recently developed a novel human WS model using WRN-deficient human mesenchymal stem cells (MSCs). This model recapitulates many phenotypic features of WS. Based...
Importance and uses of ascorbate in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
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Naive pluripotent stem cells are located within the epiblast of mature blastocysts. These primitive “ground-state” cells may be cultured in vitro using specialized media and small molecule inhibitors.
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Organoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.
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Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.
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