In order to achieve 2% BSA, dissolve 100 mL of PBS in 2 G of BSA. So, dissolving 20 G of BSA in 1000 mL of PBS would give 2% BSA.
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A5611
Bovine Serum Albumin
Cohn Fraction V, lyophilized powder, ≥96% (agarose gel electrophoresis)
Synonim(y):
Albumin bovine serum, BSA, Bovine albumin
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
10 g | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 2090,00 zł |
Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352202
EC Number:
232-936-2
NACRES:
NA.25
MDL number:
eCl@ss:
32160409
Biological source:
bovine
Assay:
≥96% (agarose gel electrophoresis)
Form:
lyophilized powder
Technique(s):
immunohistochemistry: suitable
2090,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócbiological source
bovine
Quality Segment
assay
≥96% (agarose gel electrophoresis)
form
lyophilized powder
packaging
poly bottle of
origin
USA origin
technique(s)
immunohistochemistry: suitable
pH
4.8-7.5
solubility
H2O: soluble 40 mg/mL, clear to slightly hazy, yellow
UniProt accession no.
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
General description
Serum albumins are soluble constituents of the circulatory system. Bovine serum albumin (BSA) is homologous to human serum albumin (HSA). BSA consists of three homologous domains (I, II, III), that are separated into nine loops by disulfide bonds. Serum albumin acts as a depot protein.
Application
Bovine Serum Albumin has been used:
- for in vitro high molecular weight (HMW)-species formation assay
- for immunohistochemistry
- in Escherichia coli quantitative polymerase chain reaction (qPCR) analysis
Biochem/physiol Actions
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Preparation Note
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
This is a cold alcohol fractionated albumin that is produced by Sigma-Aldrich with a stricter adherence to the original Cohn method of fractionation than other commercially available bovine albumins.
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Ta pozycja | |||
|---|---|---|---|
| description Cohn Fraction V, lyophilized powder, ≥96% (agarose gel electrophoresis) | description lyophilized powder, essentially IgG free, ≥96% (agarose gel electrophoresis) | description heat shock fraction, lyophilized powder, essentially fatty acid free, ≥98% (agarose gel electrophoresis) | description heat shock fraction, low endotoxin, pH 7, ≥98% |
| biological source bovine | biological source bovine | biological source bovine | biological source bovine |
| technique(s) immunohistochemistry: suitable | technique(s) immunofluorescence: suitable, flow cytometry: suitable | technique(s) cell culture | mammalian: suitable | technique(s) ELISA: suitable, electrophoresis: suitable, western blot: suitable |
| assay ≥96% (agarose gel electrophoresis) | assay ≥96% (agarose gel electrophoresis) | assay ≥98% (agarose gel electrophoresis) | assay ≥98% |
| origin USA origin | origin USA origin | origin USA origin | origin USA origin |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
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Klasa składowania
11 - Combustible Solids
Co się dzieje?
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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How can I prepare 1L of PBS with 2% of BSA
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