A6191

Antipain dihydrochloride from microbial source

protease inhibitor

• Synonym(s): N-(Nα-Carbonyl-Arg-Val-Arg-al)-Phe
• Empirical Formula (Hill Notation): C₂₇H₄₄N₁₀O₆ · 2 HCl
• CAS Number: 37682-72-7
• Molecular Weight: 677.62
• Numer MDL: MFCD00135957
• Identyfikator substancji w PubChem: 24891067

Properties

• pochodzenie biologiczne: Streptomyces sp.
• Poziom jakości: 300
• forma: lyophilized powder
• siła działania: 1.4 μM K_i
• rozpuszczalność: H₂O: 50 mg/mL; 1-butanol: soluble; 1-propanol: soluble; DMSO: soluble; ethanol: soluble; methanol: soluble
• Tryb działania: enzyme | inhibits
• temp. przechowywania: −20°C
• SMILES string: Cl[H].Cl[H].[H]C(=O)C(CCCNC(N)=N)NC(=O)[C@@H](NC(=O)[C@H](CCCNC(N)=N)NC(=O)NC(Cc1ccccc1)C(O)=O)C(C)C
• InChI: 1S/C27H44N10O6.2ClH/c1-16(2)21(23(40)34-18(15-38)10-6-12-32-25(28)29)37-22(39)19(11-7-13-33-26(30)31)35-27(43)36-20(24(41)42)14-17-8-4-3-5-9-17;;/h3-5,8-9,15-16,18-21H,6-7,10-14H2,1-2H3,(H,34,40)(H,37,39)(H,41,42)(H4,28,29,32)(H4,30,31,33)(H2,35,36,43);2*1H/t18?,19-,20?,21-;;/m0../s1
• InChI key: YAHXZYICKJUJEO-BXLPLHKWSA-N

Description

Opis ogólny

Chemical structure: peptide

Zastosowanie

Antipain dihydrochloride from microbial source has been used in the preparation of adipocyte proteins for western blotting and in protease inhibitor cocktail for isolating nuclear extracts for gelshift analysis.

Concentrations for 50% inhibition (μg/ml):
papain, 0.16
trypsin, 0.26
cathepsin A, 1.19
cathepsin B, 0.59
cathepsin D, 125
plasmin, >93
chymotrypsin and pepsin, >250
It also has been reported to inhibit calpain I, (porcine) with K_i = 1.4 μM

Opakowanie

1, 5, 25, 100 mg in glass bottle

Działania biochem./fizjol.

Antipain dihydrochloride also inhibits the action of papain and cathespsin B.

Reversible inhibitor of serine/cysteine proteases and some trypsin-like serine proteases. Its action resembles leupeptin; however, its plasmin inhibition is less and its cathepsin A inhibition is more than that observed with leupeptin. Chronic administration of antipain can reduce the frequency of chemically induced transformation in BALB/c-/3T3 cells.

Uwaga dotycząca przygotowania

Solubility testing at 50 mg/ml in water yields a clear to slightly hazy yellow solution. It is reportedly soluble in methanol, water, and DMSO; less soluble in ethanol, butanol, and propanol; insoluble in benzene, hexane, and chloroform.⁸ A stock solution in water or buffer is stable for about a month at -20 °C.

Dilute solutions should be stored on ice and kept for only a day because of the terminal aldehyde, which is subject to oxidation and racemization.

Stock solutions in water or buffer stable for 1 week at 4 °C, 1 month at −20 °C.

Safety Information

• Kod klasy składowania: 13 - Non Combustible Solids
• WGK: WGK 3
• Temperatura zapłonu °F: Not applicable
• Temperatura zapłonu °C: Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)