• A6191
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Antipain dihydrochloride from microbial source

protease inhibitor

Empirical Formula (Hill Notation):
C27H44N10O6 · 2 HCl
CAS Number:
Molecular Weight:
Numer MDL:
Identyfikator substancji w PubChem:

pochodzenie biologiczne

Streptomyces sp.

Poziom jakości


lyophilized powder

siła działania

1.4 μM Ki


H2O: 50 mg/mL
1-butanol: soluble
1-propanol: soluble
DMSO: soluble
ethanol: soluble
methanol: soluble

Tryb działania

enzyme | inhibits

temp. przechowywania


SMILES string




InChI key


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Opis ogólny

Chemical structure: peptide


Antipain dihydrochloride from microbial source has been used in the preparation of adipocyte proteins for western blotting and in protease inhibitor cocktail for isolating nuclear extracts for gelshift analysis.
Concentrations for 50% inhibition (μg/ml):
papain, 0.16
trypsin, 0.26
cathepsin A, 1.19
cathepsin B, 0.59
cathepsin D, 125
plasmin, >93
chymotrypsin and pepsin, >250
It also has been reported to inhibit calpain I, (porcine) with Ki = 1.4 μM


1, 5, 25, 100 mg in glass bottle

Działania biochem./fizjol.

Antipain dihydrochloride also inhibits the action of papain and cathespsin B.
Reversible inhibitor of serine/cysteine proteases and some trypsin-like serine proteases. Its action resembles leupeptin; however, its plasmin inhibition is less and its cathepsin A inhibition is more than that observed with leupeptin. Chronic administration of antipain can reduce the frequency of chemically induced transformation in BALB/c-/3T3 cells.

Uwaga dotycząca przygotowania

Solubility testing at 50 mg/ml in water yields a clear to slightly hazy yellow solution. It is reportedly soluble in methanol, water, and DMSO; less soluble in ethanol, butanol, and propanol; insoluble in benzene, hexane, and chloroform.8 A stock solution in water or buffer is stable for about a month at -20 °C.

Dilute solutions should be stored on ice and kept for only a day because of the terminal aldehyde, which is subject to oxidation and racemization.
Stock solutions in water or buffer stable for 1 week at 4 °C, 1 month at −20 °C.

Kod klasy składowania

13 - Non Combustible Solids



Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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