The Casein used to prepare this product is a mixture of Alpha-s1, Alpha-s2, Beta, and Kappa Casein. Combined, these forms of casein have a molecular weight range of 19 - 25.2 kDa. The Fluorescein isothiocyanate I isomer has a molecular weight of 389.38. The ratio of casein to FITC is approximately 11:1. A confirmed molecular weight has not been established.
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C0528
Casein fluorescein isothiocyanate from bovine milk
Type III, essentially salt-free, lyophilized powder
Synonim(y):
FITC-casein
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Type III
Quality Segment
form
essentially salt-free, lyophilized powder
extent of labeling
≥50 μg FITC per mg solid
solubility
water: 5 mg/mL, clear to hazy
storage temp.
2-8°C
Application
highly sensitive protease substrate
Casein fluorescein isothiocyanate from bovine milk has been used:
- in accessing zona pellucida (ZP) and perivitelline space (PVS) to evaluate changes induced by protease treatment
- to make a reference line in immunochromatographic devices
- in running gel for the documentation of proteinases
Biochem/physiol Actions
Casein fluorescein isothiocyanate is mainly used as a substrate in estimating the protease activity of psychrotrophic bacteria. It is also used as a substrate for determining the activity of bacteria in milk.
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Ta pozycja | |||
|---|---|---|---|
| description Type III, essentially salt-free, lyophilized powder | description Type II, essentially salt-free, lyophilized powder | description average mol wt 60,000-76,000 | description average mol wt 70,000, (FITC:Glucose = 1:250) |
| solubility water: 5 mg/mL, clear to hazy | solubility water: 5 mg/mL, clear to hazy, yellow to orange | solubility H2O: 25 mg/mL, clear to slightly hazy, yellow-orange | solubility H2O: 50 mg/mL, hazy, orange to very deep orange |
| form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder | form powder | form powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| extent of labeling ≥50 μg FITC per mg solid | extent of labeling 20-50 μg FITC per mg solid | extent of labeling 0.003-0.020 mol FITC per mol glucose | extent of labeling ~0.004 mol/mol FITC glucose |
| type Type III | type Type II | type - | type - |
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Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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What's the mwt of fitc-casein in kda?
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