Przejdź do zawartości
Merck

Przejdź do

C2888

Acetylcholinesterase from Electrophorus electricus (electric eel)

Type V-S, lyophilized powder, ≥1,000 units/mg protein

Synonim(y):

AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl

Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.

Wybierz wielkość

Zmień widok
Rozmiar/SKUDostępnośćCena netto
500 units
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
631,00 zł
1000 units
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
1710,00 zł
5000 units
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
3830,00 zł

Informacje o tej pozycji

Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
232-559-3
MDL number:
Numer WE:
Specific activity:
≥1,000 units/mg protein
Biological source:
Electrophorus electricus

631,00 zł


Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności

​
Złóż zamówienie na produkt na zamówienie
Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomóc


biological source

Electrophorus electricus

Quality Segment

type

Type V-S

form

lyophilized powder

specific activity

≥1,000 units/mg protein

mol wt

280 kDa

composition

Protein, ≥45% biuret

pI 

~5.3

solubility

Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5)

storage temp.

−20°C

General description

Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)

Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.

Application

Acetylcholinesterase from Electrophorus electricus (electric eel) has been used:
  • in acetylcholinesterase activity assay
  • to study its effects on Xenopus embryonic hippocampal cells
  • to study the effect of acetylcholine inhibitors on development and plasticity

Biochem/physiol Actions

Acetylcholinesterase (AChE) exhibits its activity against acetylcholine in the neuromuscular junctions and synaptic cleft of cholinergic neurons. It participates in the nitric oxide signaling pathway.
Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid.

Physical form

Lyophilized powder containing Tris buffer salts

Analysis Note

The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.

Other Notes

One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.

Disclaimer

For dilute solutions (<1 mg/ml), add 1 mg/ml BSA to stabilize. Solutions containing at least 1 mg protein/ml in dilute neutral phosphate buffer are stable at least 6 months refrigerated.
Ta strona może zawierać tekst przetłumaczony maszynowo.

Porównaj podobne pozycje

Wyświetl pełne porównanie

Pokaż różnice

1 of 1

Ta pozycja
C1682C7512A2580
description

Type V-S, lyophilized powder, ≥1,000 units/mg protein

description

recombinant, expressed in HEK 293 cells, lyophilized powder, ≥1,000 units/mg protein (BCA)

description

lyophilized powder, ≥10 units/mg protein

description

lyophilized powder, ≥10 units/mg protein (Bradford)

biological source

Electrophorus electricus

biological source

-

biological source

-

biological source

-

specific activity

≥1,000 units/mg protein

specific activity

≥1,000 units/mg protein (BCA)

specific activity

≥10 units/mg protein

specific activity

≥10 units/mg protein (Bradford)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

solubility

Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5)

solubility

-

solubility

cold water: 60 U/mL

solubility

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

280 kDa

mol wt

64.6 kDa

mol wt

tetramer 440 kDa

mol wt

-


Still not finding the right product?


Klasa składowania

11 - Combustible Solids

Co się dzieje?

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

PPE (środki ochrony indywidualnej)

Eyeshields, Gloves, type N95 (US)



Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Dokumenty section.

Proszę o kontakt, jeśli potrzebna jest pomoc Obsługa Klienta

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów




Questions

1–2 of 2 Questions  
  1. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  2. Are solutions of Product C2888, Acetylcholinesterase from Electrophorus electricus (electric eel), harmed by freezing?

    1 answer
    1. Yes, solutions may be harmed by freezing. The data sheet for Product C2888 indicates that solutions stabilized by the addition of BSA are stable when kept refrigerated for up to six months. A customer has reported to us that they tried freezing a solution to which BSA was added; they found that the solution had a 40 percent loss of activity after four months in the freezer. So our best recommendation is that solutions be stabilized with BSA and kept refrigerated, not frozen.

      Helpful?

Reviews

No rating value

Active Filters