Merck
Wszystkie zdjęcia(1)

C5301

Sigma-Aldrich

Monoclonal Anti-Cytokeratin Peptide 8 antibody produced in mouse

clone M20, ascites fluid

Synonim(y):
Anti-CK8, Anti-KO, Anti-CK-8, Anti-CYK8, Anti-CARD2, Anti-K2C8, Anti-K8
Numer MDL:
NACRES:
NA.41

Poziom jakości

200

pochodzenie biologiczne

mouse

białko sprzężone

unconjugated

forma przeciwciała

ascites fluid

antibody product type

primary antibodies

klon

M20, monoclonal

masa cząsteczkowa

antigen 52 kDa

zawiera

15 mM sodium azide

species reactivity

rabbit, canine, human, feline, bovine

technique(s)

immunohistochemistry (frozen sections): suitable
indirect immunofluorescence: 1:200 using frozen sections of human or animal tissue
western blot: suitable

izotyp

IgG1

numer dostępu UniProt

transport

dry ice

temp. przechowywania

−20°C

Gene Information

human ... KRT8(3856)

Opis ogólny

Cytokeratin is a 52.5kD cytoplasmic structural protein that belongs to type II, neutral-to-basic subfamily. It consists of 29 different proteins which is the characteristic of epithelial and trichocytic cells. Monoclonal anti-cytokeratin peptide 8 antibody can be used as a marker for epithelial cell differentiation as well as a tool for tumor identification and classification. It can also be used in western blotting. Mouse anti-cytokeratin peptide 8 antibody reacts specifically with various human simple and complex epithelia like- liver, intestine, pancreas, urinary bladder, salivary gland, thyroid, prostate, mesothel, and placenta but not with stratified squamous epithelia. The product also does not react with non-epithelial tissues (exceptions-certain smooth muscle cells). This product has also shown cross reactivity for cytokeratin of rabbit, cow, dog, and cat.Monoclonal Anti-Cytokeratin 8 (mouse IgG1 isotype) is derived from the M20 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a cytokeratin preparation purified from the human breast carcinoma cell line MCF7.

Specyficzność

Mouse anti-cytokeratin peptide 8 antibody reacts specifically with various human simple and complex epithelia like- liver, intestine, pancreas, urinary bladder, salivary gland, thyroid, prostate, mesothel, and placenta but not with stratified squamous epithelia. The product also does not react with non-epithelial tissues (exceptions-certain smooth muscle cells). This product has also shown cross reactivity for cytokeratin of rabbit, cow, dog, and cat.

Immunogen

cytokeratin from human breast carcinoma cell line MCF7.

Zastosowanie

Monoclonal anti-cytokeratin peptide 8 antibody has been used in
  • immunofluorescence microscopy
  • immunohistochemistry
  • dot blotting
  • protein preparation from bovine epithelial cells
  • immunofluorescence
  • western blotting

Działania biochem./fizjol.

Monoclonal anti-cytokeratin peptide 8 antibody can be used as a marker for epithelial cell differentiation as well as a tool for tumor identification and classification. It can also be used in western blotting.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Kod klasy składowania

12 - Non Combustible Liquids

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Certyfikat analizy

Wprowadź numer partii, aby wyszukać certyfikat analizy (COA).

Świadectwo pochodzenia

Wprowadź numer partii, aby wyszukać świadectwo pochodzenia (COO).

Mingxi Hua et al.
Journal of cell science, 125(Pt 23), 5800-5810 (2012-09-15)
The correct functioning of hepatocytes requires the establishment and maintenance of hepatocyte polarity. However, the mechanisms regulating the generation of hepatocyte polarity are not completely understood. The differentiation of human fetal hepatic progenitor cells (hFHPCs) into functional hepatocytes provides a
S Portet et al.
Cytometry, 35(3), 203-213 (1999-03-19)
In the MCF7 human breast cancer cell line, several patterns of cytokeratin networks are observed, depending on the intracellular localization. Our hypothesis is that architectural variations of cytokeratin networks depend on local tensions or forces appearing spontaneously in the cytoplasm.
G N Van Muijen et al.
Laboratory investigation; a journal of technical methods and pathology, 57(4), 359-369 (1987-10-01)
Tissues from human fetuses (12 to 14 weeks) were studied by using immunohistochemical methods, with special emphasis on coexpression of intermediate filaments. Well-characterized antibodies, monoclonal as well as polyclonal were used. Indirect immunoperoxidase staining disclosed simultaneous expression of cytokeratin and
Myoglobin: a promising exogenous reference marker using in proteomics analysis
Wang T, et al.
Food Science and Biotechnology, 22, 393-398 (2013)
A Bader et al.
Differentiation; research in biological diversity, 68(1), 31-43 (2001-10-31)
In the vertebrate embryo the heart is the first organ to form. Embryonic and extra-embryonic tissues are supposed to contribute to cardiac lineage commitment before and during gastrulation in a paracrine fashion. Evidence has accumulated that factors secreted by the

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