C6286

Cathepsin B from bovine spleen

lyophilized powder, ≥10 units/mg protein

• Synonim(y): Cathepsin B₁
• Numer CAS: 9047-22-7
• UNSPSC Code: 12352204
• NACRES: NA.32
• Numer WE: 3.4.22.1 (BRENDA, IUBMB)
• MDL number: MFCD02662345
• Specific activity: ≥10 units/mg protein
• Biological source: bovine spleen

Właściwości

• biological source: bovine spleen
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥10 units/mg protein
• composition: Protein, ≥30% biuret
• UniProt accession no.: P07688
• storage temp.: −20°C
• Gene Information: cow ... CTSB(281105)

Opis

General description

Cathepsin B is a lysosomal enzyme that comprises a light chain (Lys1–Arg49) and a heavy chain (Val50–Thr253). It belongs to cysteine family C1.

Application

Cathepsin B from bovine spleen has been used in cleavage assay before high-performance liquid chromatography-mass spectrometry (HPLC-MS) analysis. It has also been used in the enzyme inhibition assays with protease inhibitors RfIP1 and ruthenium metalloarenes.

Biochem/physiol Actions

Cathepsin B displays an endopeptidase and peptidyl dipeptidase activities. It may be associated with the pathophysiology of tumors. Cathepsin B is also regarded as a prominent protease in Leishmaniasis. It is overactivated in muscular dystrophy, pulmonary emphysema, and bone resorption.

Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.

Found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells.

Physical form

Lyophilized powder containing sodium phosphate, sodium chloride and ~6% EDTA as stabilizer.

Other Notes

One unit will hydrolyze 1 μmole of Z-lysine p-nitrophenyl ester per min at pH 5.0 at 25 °C.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• Co się dzieje?: WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• PPE (środki ochrony indywidualnej): Eyeshields, Gloves, type N95 (US)