C6788

4-Chloro-1-naphthol

peroxidase substrate, tablet, suitable for use in immunoblotting procedures

• Synonim(y): 4-CN, 4-chloronaphthalen-1-ol
• Wzór liniowy: ClC₁₀H₆OH
• Numer CAS: 604-44-4
• Masa cząsteczkowa: 178.61
• NACRES: NA.83
• PubChem Substance ID: 57654035
• UNSPSC Code: 12352204
• EC Number: 210-068-5
• MDL number: MFCD00003974
• Beilstein/REAXYS Number: 509750

Właściwości

• Nazwa produktu: 4-Chloro-1-naphthol, tablet
• form: tablet
• Quality Segment: 200
• technique(s): protein detection: suitable
• mp: 118-121 °C (lit.)
• solubility: methanol: 10 mL, clear, colorless
• storage temp.: −20°C
• SMILES string: Oc1ccc(Cl)c2ccccc12
• InChI: 1S/C10H7ClO/c11-9-5-6-10(12)8-4-2-1-3-7(8)9/h1-6,12H
• InChI key: LVSPDZAGCBEQAV-UHFFFAOYSA-N

Opis

Application

4-Chloro-1-naphthol has been used as a substrate in enzyme-linked immunospot (ELISpot) assay for detection of the spots. It has also been used as a precipitating chromogenic substrate for visualizing the antigen−antibody-reactive bands following western blotting.

Biochem/physiol Actions

4-Chloro-1-Naphthol is a peroxidase substrate suitable for use in immunoblotting procedures. This substrate produces an insoluble end product that is blue in color and can be observed visually.

Preparation Note

Contains 30 mg substrate per tablet.

Dissolve 1 tablet in 10 ml of methanol. Add 2 ml of methanol stock solution to 10 ml of triethanolamine buffered saline, pH 7.5. Add 5 μl of fresh 30% hydrogen peroxide (Sigma Product No. H-1009) immediately prior to use.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• Co się dzieje?: WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• PPE (środki ochrony indywidualnej): dust mask type N95 (US), Eyeshields, Gloves