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A549 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86012804, human lung (carcinoma), Epithelial

Synonim(y):

A-549 Cells, A549 Cells, NCI-A549 Cells

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Informacje o tej pozycji

UNSPSC Code:
41106514
eCl@ss:
32160903
Biological source:
human lung (carcinoma)
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Hypotriploid
Morphology:
Epithelial
Manufacturer/tradename:
ECACC
Informacje o cenach i dostępności nie są obecnie dostępne.
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biological source

human lung (carcinoma)

manufacturer/tradename

ECACC

packaging

tube of 5 μg 86012804-DNA-5UG, pkg of vial of cells 86012804-1VL

growth mode

Adherent

karyotype

Hypotriploid

morphology

Epithelial

products

Lecithin, High amounts of de-saturated fatty acids.

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Application

Enzymatic studies
Human A549 cell line has been used:
  • to study the ability of 2-(2,4-dihydroxyphenyl)thieno-1,3-thiazin-4-one (BChTT) to inhibit cancer cell proliferation and identify mechanisms involved in its actions
  • to study the effects of insulin and insulin-like growth factor 1 (IGF1) on proliferation and apoptosis in A549 cells
  • to culture A549 cells in different media over a long term and identify conditions for promoting alveolar type II differentiation characteristics in A549 cells

Biochem/physiol Actions

Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.
Human Caucasian lung carcinoma
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14

Preparation Note

Ham′s F12K + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% Trypsin or tTrypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
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HLA003ATG002ATG005
A549 Cell Line human NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86012804, human lung (carcinoma), Epithelial

A549 Cell Line human

Panel linii komórkowych A549 HLA

Sigma-Aldrich

HLA003

Panel linii komórkowych A549 HLA

Panel linii komórkowych A549 z antygenem EGFR

Sigma-Aldrich

ATG002

Panel linii komórkowych A549 z antygenem EGFR

Panel antygenów A549 EGFRvIII Linie komórkowe

Sigma-Aldrich

ATG005

Panel antygenów A549 EGFRvIII Linie komórkowe

description

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86012804, human lung (carcinoma), Epithelial

description

-

description

-

description

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

karyotype

Hypotriploid

karyotype

-

karyotype

-

karyotype

-

packaging

pkg of vial of cells 86012804-1VL, tube of 5 μg 86012804-DNA-5UG

packaging

-

packaging

-

packaging

-

biological source

human lung (carcinoma)

biological source

human lung (Carcinoma)

biological source

human lung (carcinoma)

biological source

human lung (carcinoma)

products

Lecithin, High amounts of de-saturated fatty acids.

products

-

products

-

products

-

receptors

Not specified

receptors

-

receptors

-

receptors

-

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Lot/Batch Number

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Questions

1–9 of 9 Questions  

  1. What HLA-ABC phenotype do A549 cells show?

    1 answer

    1. Please view the article in the link below for more information regarding the phenotype of this cell line:
      https://www.culturecollections.org.uk/media/126668/jim-plos-one-article-a549.pdf

      Please visit the ECACC website for additional documentation and information regarding this cell line:
      https://www.culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=86012804&collection=ecacc_gc

      Helpful?

  2. What is cell line?

    1 answer

    1. Hello. Human A549 cell line is a human lung carcinoma that was derived from a 58 year old Caucasian male.

      Helpful?

  3. Are my Cell Line cells mycoplasma tested?

    1 answer

    1. ECACC routinely tests all manufactured cell banks for mycoplasma.

      Helpful?

  4. How many Cell Line cells are in the vial?

    1 answer

    1. Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule.  For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.

      Helpful?

  5. What medium should I use when I thaw the Cell Line cells?

    1 answer

    1. The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.

      Helpful?

  6. What passage are my Cell Line cells?

    1 answer

    1. If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).

      Helpful?

  7. Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?

    1 answer

    1. If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.

      Helpful?

  8. How do I handle Cell Line frozen cells upon arrival?

    1 answer

    1. Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.

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  9. Are the Cell Line cells tested for viral pathogens?

    1 answer

    1. HPA Cultures does not perform any viral testing on the cell lines.

      Helpful?

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