SW 48 Human Colon, ECACC 89012702

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89012702, human colon, Epithelial

• UNSPSC Code: 41106514
• eCl@ss: 32160703
• Biological source: human colon
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: 2n = 46
• Morphology: Epithelial
• Manufacturer/tradename: ECACC

Właściwości

• Nazwa produktu: SW 48, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89012702, human colon, Epithelial
• biological source: human colon
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 89012702-DNA-5UG, pkg of vial of cells 89012702-1VL
• growth mode: Adherent
• karyotype: 2n = 46
• morphology: Epithelial
• products: Carcinoembryonic antigen (CEA)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Opis

Application

Tumourigenicity studies

Biochem/physiol Actions

Dane STR-PCR: Amelogenina: X
CSF1PO: 9,10
D13S317: 11,12
D16S539: 11,13
D5S818: 10,14
D7S820: 9,10
THO1: 6,9.3
TPOX: 8
vWA: 18,20

Komórki wytwarzają niski poziom CEA: 0,6ng/1 000 000 komórek w ciągu 10 dni. Komórki są nowotworotwórcze u nagich myszy. Wyhodowane z wrzodziejącego guza IV stopnia otaczającego jelito 82-letniej kobiety rasy kaukaskiej. HLA = A32, A33, B27, B35

Ludzki gruczolakorak okrężnicy

Preparation Note

L15 + 2mM Glutaminy + 10% Płodowej Surowicy Bydlęcej (FBS).

Rozdzielić subkonfluentne kultury (70-80%) w stosunku 1:3 do 1:6, tj. wysiać 2-4x10 000 ^komórek/cm2 przy użyciu 0,25% trypsyny/EDTA; Bez_CO2; 37°C.

Other Notes

W przypadku przesyłek w regionie Azji i Pacyfiku mogą obowiązywać dodatkowe opłaty za transport i obsługę. Więcej informacji można uzyskać u lokalnego przedstawiciela działu obsługi klienta.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

Disclaimer

This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.

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