SCC-4 Human Mouth, ECACC 89062002

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89062002, human tongue, squamous cell carcinoma. Epithelial morphology.

• UNSPSC Code: 41106514
• eCl@ss: 32160703
• Biological source: human mouth
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Epithelial
• Manufacturer/tradename: ECACC

Właściwości

• Nazwa produktu: SCC-4, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89062002, human tongue, squamous cell carcinoma. Epithelial morphology.
• biological source: human mouth
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 89062002-DNA-5UG, pkg of vial of cells 89062002-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Epidermal keratins; 40 kD Keratin
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Opis

Biochem/physiol Actions

Ludzki rak płaskonabłonkowy języka

Pochodzi z ludzkiego raka płaskonabłonkowego (SCC) języka od 55-letniego mężczyzny. Stwierdzono, że komórki SCC-4 tworzą kolonie w pożywce półstałej i nie są indukowane do różnicowania przez pozbawienie zakotwiczenia. Wzrost jest wzmocniony przez zastosowanie warstwy komórek 3T3 swiss.

Preparation Note

DMEM:HAMS F12 (1:1) + 2 mM glutaminy + 10% płodowej surowicy bydlęcej (FBS) + 0,4 μg/ml hydrokortyzonu

Rozdzielenie konfluentnych kultur w stosunku 1:3 do 1:4, tj. wysiew 3-5 x 10 000 ^komórek/cm2 przy użyciu trypsyny/EDTA; 5%_CO2; 37°C.

Other Notes

W przypadku przesyłek w regionie Azji i Pacyfiku mogą obowiązywać dodatkowe opłaty za transport i obsługę. Więcej informacji można uzyskać u lokalnego przedstawiciela działu obsługi klienta.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

Disclaimer

This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.

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