59M

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Synonim(y): Ogniwa OAW 59M, Ogniwa OAW59M
• UNSPSC Code: 41106514
• Biological source: human ovary
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Epithelial
• Manufacturer/tradename: ECACC

Właściwości

• biological source: human ovary
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 89081802-DNA-5UG, pkg of vial of cells 89081802-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Opis

Application

Tumourigenicity studies

Biochem/physiol Actions

Ludzki nabłonek guza jajnika

Pochodzi od pacjentki z rakiem jajnika i jest pochodzenia płynu puchlinowego.

Preparation Note

DMEM + 2 mM glutaminy + 1 mM pirogronianu sodu (NaP) + 20 IU/l insuliny bydlęcej + 10% płodowej surowicy bydlęcej (FBS).

Rozdzielić subkonfluentne hodowle (70-80%) 1:3, tj. wysiać 4x10 000 ^komórek/cm2 przy użyciu 0,25% trypsyny lub trypsyny/EDTA; 5%_CO2; 37°C.

Other Notes

Depozytariusz i pomysłodawca: Dr A Wilson, Laboratorium Badań Onkologicznych, Szpital Miejski w Derby

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

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