Przejdź do
CLL1136
U2OS Cells GFP-NUP98
human female bone (Disease source: Osteosarcoma)
Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.
Wybierz wielkość
Zmień widok
| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
1 vial | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 22 420,00 zł |
Informacje o tej pozycji
UNSPSC Code:
41106514
NACRES:
NA.81
Biological source:
human female bone (Source Disease: Bone osteosarcoma)
22 420,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomócNazwa produktu
U2OS Cells GFP-NUP98,
biological source
human female bone (Source Disease: Bone osteosarcoma)
Quality Segment
OMIM accession no.
storage temp.
−196°C
Gene Information
human ... NUP98(4928)
General description
U2OS GFP-NUP98 are bone osteosarcoma cells from a human 15 year old caucasian female having a ZFN modification creating a GFP-NUP98 transgene expressed from the endogenous NUP98 gene locus.
This cell line was derived from ATCC Catalog No. HTB-96.
This cell line was derived from ATCC Catalog No. HTB-96.
Application
U2OS Cells have been used to assess cell proliferation using Alamar Blue and live cell imaging. This product is a human U2OS cell line in which the genomic NUP98 gene has been endogenously tagged with a 3XFLAG™ epitope and a Green Fluorescent Protein (GFP) gene using CompoZr® Zinc Finger Nuclease technology. Integration resulted in endogenous expression of a fusion protein in which the 3XFLAG is attached to the N-terminus of GFP and the GFP is attached to the N-terminus of NUP98. Fluorescence imaging shows characteristic NUP98 nuclear expression. This stable cell line was expanded from a single clone. The target′s gene regulation and corresponding protein function are preserved in contrast to cell lines with overexpression via an exogenous promoter.
To learn more, please visit the Cellular Reporter Cell Line webpage
To learn more, please visit the Cellular Reporter Cell Line webpage
Features and Benefits
Zinc finger nuclease (ZFN)-mediated targeted integration of a fluorescent GFP tag just behind the start codon of the NUP98 gene on chromosome 11p15.4 to create a cell line exhibiting stable expression of the transgene tagged with GFP on the N-terminus of the protein.
The U2OS cells are adherent, with a doubling time of approx. 29 hours.
The U2OS cells are adherent, with a doubling time of approx. 29 hours.
Preparation Note
Media Renewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3-1:6
The base medium for this cell line is McCoy′s 5A Medium Modified, Cat. No. M9309. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%.
Cell freezing medium-DMSO 1X, Cat. No. C6164.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3-1:6
The base medium for this cell line is McCoy′s 5A Medium Modified, Cat. No. M9309. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%.
Cell freezing medium-DMSO 1X, Cat. No. C6164.
Analysis Note
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.
Legal Information
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany
Ta strona może zawierać tekst przetłumaczony maszynowo.
1 of 1
Ta pozycja | |||
|---|---|---|---|
| description - | description - | description - | description - |
| biological source human female bone (Source Disease: Bone osteosarcoma) | biological source human female bone (Source Disease: Osteosarcoma) | biological source human female bone (Source Disease: Osteosarcoma) | biological source human female bone (Source Disease: Osteosarcoma) |
| storage temp. −196°C | storage temp. −196°C | storage temp. −196°C | storage temp. −196°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| Gene Information human ... NUP98(4928) | Gene Information human ... beta actin(60) | Gene Information human ... lamin B1(4001) | Gene Information human ... HMGA1(3159) |
| OMIM accession no. | OMIM accession no. | OMIM accession no. | OMIM accession no. |
Klasa składowania
10 - Combustible liquids
Co się dzieje?
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.