BSL-1 applies to this product. This line is a SMAD4 knockout derivative of SW48 (ATCC CCL-231) created with CompoZr ZFN. Non-viral genome editing via plasmid/mRNA transfection is indicated; viral vectors have not been utilized. Product-specific testing includes mycoplasma, sterility, post-freeze viability, STR identity, and PCR species confirmation. Testing for HIV-1, HBV, HCV, or HTLV-1 is not listed. For the parental SW48 line, COA reports HIV and HBV not detected; data for HCV or HTLV-1 are not identified. Please see the following link to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/134/271/clls1010dat.pdf
Przejdź do
CLLS1010
SW48 Cells SMAD4 -/-
Human female colon, colorectal adenocarcinoma
Synonim(y):
CLL1008
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
1 set | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 20 800,00 zł |
Informacje o tej pozycji
UNSPSC Code:
41106514
NACRES:
NA.81
Biological source:
human female colon (Source Disease: Colorectal adenocarcinoma)
20 800,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócNazwa produktu
SW48 Cells SMAD4 -/-,
biological source
human female colon (Source Disease: Colorectal adenocarcinoma)
Quality Segment
OMIM accession no.
storage temp.
−196°C
Gene Information
human ... SMAD4(4089)
General description
SW48 Cells SMAD4 -/- are colorectal adenocarcinoma, epithelial cells, from a human caucasian female (aged 82 years), with a ZFN knock out modification.
This product corresponds to ATCC Cat. No. CCL-231
Application
Media Renewal changes one to two times per week
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:2 to 1:6
The base medium for this cell line is DMEM, Cat. No. D5671. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%, Sodium pyruvate, Cat. No. S8636, at a final concentration of 1 mM, and L-glutamine, Cat. No. G7513, at a final concentration of 2mM.
The cell freezing medium is DMSO 1X (Cat. No. C6164)
Features and Benefits
These SW48 cells are adherent, with a doubling time of approx. 35 hours.
Zinc finger nuclease (ZFN) knock out on chromosome 18q21.1
Analysis Note
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.
Disclaimer
This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.
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Ta pozycja | |||
|---|---|---|---|
| description - | description - | description - | description - |
| biological source human female colon (Source Disease: Colorectal adenocarcinoma) | biological source human female colon (Source disease: Colorectal adenocarcinoma) | biological source human female colon (Source Disease: Colorectal adenocarcinoma) | biological source human female colon (Source Disease: Colorectal adenocarcinoma) |
| storage temp. −196°C | storage temp. −196°C | storage temp. −196°C | storage temp. −196°C |
| Gene Information human ... SMAD4(4089) | Gene Information human ... HIF1A(3091) | Gene Information human ... BAX(581) | Gene Information human ... PTEN(5728) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| OMIM accession no. | OMIM accession no. - | OMIM accession no. | OMIM accession no. |
Klasa składowania
10 - Combustible liquids
WGK
WGK 3
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Please tell me the biosafety level (BSL) for this cell line. Are viruses being used to genetically modify these cells? Are you testing for infections with viruses such as HIV-1, HBV, HCV, and HTLV-1?
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