D6434

DMEM/F-12

With HEPES, sodium bicarbonate and sodium pyruvate, without ʟ-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

• Synonim(y): DME/Nutrient Mixture F-12 Ham, DMEM Hams F12, DME/F-12, 1:1 mixture
• UNSPSC Code: 12352207
• NACRES: NA.75
• MDL number: MFCD00217417

Właściwości

• Nazwa produktu: Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With 15 mM HEPES and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture
• Quality Segment: 400
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: L-glutamine: no; phenol red: no; NaHCO₃: yes; HEPES: 15 mM; sodium pyruvate: 0.055 g/L; glucose: 3.15 g/L
• shipped in: ambient
• storage temp.: 2-8°C

Opis

General description

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Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium. Suitable for growth of a wide range of cell types in low serum conditions. Absence of phenol red makes this a preferred medium for clonal and stem cell culture.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used to culture:

• bovine endometrial epithelial cells
• cancer stem cells
• breast cancer cell line

Preparation Note

Supplement with 0.365 g/L L-glutamine

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS07
• Słowo ostrzegawcze: Warning
• Kody zagrożeń: H317
• Ostrzeżenia: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Klasa składowania: 12 - Non Combustible Liquids
• Co się dzieje?: WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable