The volume of the container is 2 mL. Reconstitution of this product should be performed using 0.2 µm filtered 20 mM Tris, pH 7.0, to prepare a stock solution of >= 25 ug/mL. This stock solution may be diluted immediately before use to achieve the final working concentration. Additional filtration is not recommended, as it may result in product loss due to adsorption onto the filter membrane.
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F0291
Fibroblast Growth Factor-Basic, human
≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonim(y):
Fibroblast Growth Factor-Basic human, FGF2, bFGF, hBFGF
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
25 μg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 2100,00 zł |
Informacje o tej pozycji
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥97% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
16.0 kDa
Impurities:
≤1.00 EU/μg
2100,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócNazwa produktu
hBFGF, FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture
biological source
human
Quality Segment
recombinant
expressed in E. coli
assay
≥97% (SDS-PAGE)
form
lyophilized powder
potency
≤-0.6 ng/mL
mol wt
16.0 kDa
packaging
pkg of 4X25 μg, pkg of 25 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1.00 EU/μg
color
white to faint yellow cast
solubility
water: soluble 0.025 mg, clear, colorless to faintly yellow
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... FGF2 (2247)
General description
Fibroblast growth factor-basic (FGF-Basic) is found in basement membranes and sub-endothelial extracellular matrix. FGF-Basic is induced early in the development of the embryo where it is necessary (but not sufficient) for pluripotency and self-renewal of embryonic stem cells (ESC) and lineage defined stem cells; wherein it functions to support proliferation and inhibit differentiation. Self-renewal of hESC depends upon activation of the Activin/Nodal/Smad2,3 branch and suppression of the BMP/GDF/Smad5 branch of the TGFβ-family cell signaling program. FGF-Basic is a competence factor for Nodal support of pluripotency. Activin-A is necessary and sufficient to maintain stemness. It induces the expression of both Nodal and FGF-Basic in human ES cells.
Application
Physical form
Lyophilized from 0.2 μm filtered solution in 20 mM Tris and 1 M NaCl, pH 7.0, containing 50 μg bovine serum albumin per 1 μg BFGF.
Analysis Note
The bioactivity of FGF-Basic was measured in a fluorometric assay using the redox sensitive dye, Resazurin.
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Ta pozycja | |||
|---|---|---|---|
| description FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture | description FGF-Basic, from human, recombinant, expressed in E. coli, carrier free | description recombinant, expressed in insect cells, ≥85% (SDS-PAGE) | description The Fibroblast Growth Factor-basic (or bFGF protein) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal & endothelial cells. |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in insect cells | recombinant - |
| biological source human | biological source human | biological source human | biological source human |
| assay ≥97% (SDS-PAGE) | assay ≥97% (SDS-PAGE) | assay ≥85% (SDS-PAGE) | assay >95% (SDS-PAGE) |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) cell culture | stem cell: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level - | Quality Level 100 |
| form lyophilized powder | form lyophilized powder | form frozen liquid | form - |
Klasa składowania
11 - Combustible Solids
Co się dzieje?
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
PPE (środki ochrony indywidualnej)
Eyeshields, Gloves, type N95 (US)
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What is the volume of the vial and how do I reconstitute the powder?
1 answer-
Helpful?
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Hey! I am using this product to prepare a stem cell media. I know the concentration is 0.025mg but it has two data sheets, one that its soluble in water (0.250ml) and other that says to dissolve in 20mM Tris Base pH 7.0. How many volume should I add?
1 answer-
The solubility of this product is tested in water. It is lyophilized from a solution containing 20 mM Tris and 1M NaCl with 50 ug BSA as a stabilizer. The product data sheet recommends reconstituting in the 20 mM Tris for optimum stability and long term storage.
Helpful?
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