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F1879

Formaldehyde Dehydrogenase from Pseudomonas sp.

lyophilized powder, 1.0-6.0 units/mg solid

Synonim(y):

FDH, Formaldehyde:NAD+ oxidoreductase

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Informacje o tej pozycji

Numer CAS:
9028-84-6
UNSPSC Code:
12352204
MDL number:
MFCD00131100
Numer WE:
1.2.1.46 (BRENDA, IUBMB)
Specific activity:
1.0-6.0 units/mg solid

form

lyophilized powder

specific activity

1.0-6.0 units/mg solid

storage temp.

−20°C

General description

Formaldehyde Dehydrogenase (FDH) belongs to the medium-chain alcohol dehydrogenase family. It is encoded by the fdhA and fdhB gene in Pseudomonas aeruginosa. The FDH sequences of P. aeruginosa and P. putida are conserved and show homology. FDH exists as dimer and tetramer with zinc in their catalytic active site. The monomer corresponds to 350-400 amino-acid residues.

Application

Formaldehyde Dehydrogenase from Pseudomonas sp. has been used:

  • For standard curve generation for the demethylation-FDH assay of lysine specific demethylase 1 (LSD1) and in formaldehyde dehydrogenase (FDH) assay.
  • As a biosensor for the presence of formaldehyde in pharmaceuticals, waste water, vaccines and industrial products.
  • In coupled pectin methyl esterase (PME) enzyme assay.

Biochem/physiol Actions

Formaldehyde dehydrogenase (FDH) is an enzyme that catalyzes the oxidation of formaldehyde to formate with NAD+ as an electron acceptor.Formaldehyde dehydrogenases are involved in the biological detoxification of formaldehyde, a toxic product in the metabolism of compounds like histidine, vanillate etc. They detoxify by converting formaldehyde to carbon-di-oxide. FDH plays a crucial role in regulating protein S-nitrosation in mammalian cells by decomposing S-nitrosoglutathione. They are also involved in the regulation of nitrosothiol homeostasis by catalyzing the reduction of endogenous nitrosylating agent S-nitrosoglutathione (GSNO).

Physical form

Lyophilized powder containing ~70% stabilizers as Mg2+, Ca2+, bovine serum albumin, glycine, and lysine

Other Notes

One unit will oxidize 1.0 μmole of formaldehyde to formic acid per min at pH 7.5 at 37 °C.
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Klasa składowania

11 - Combustible Solids

Co się dzieje?

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

PPE (środki ochrony indywidualnej)

Eyeshields, Gloves, type N95 (US)

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    Certyfikaty analizy (CoA)

    Lot/Batch Number
    054K0861
    030M1407
    SLBD3584V
    SLBF8054V
    SLBP2925V

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    Questions

    1. この酵素の活性値を測定した方法について教えてください。

      1 answer

      1. The activity was found by taking the units/ml of enzyme and dividing it by the mg protein/ml of enzyme. The activity was found in an spectrophometric rate determination assay. The result found that one unit of enzyme will oxidize 1.0 micro mole of formaldehyde to formic acid per minute at pH 7.5 at 37°C.

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