F3040

Monoclonal ANTI-FLAG^® M1 antibody

clone M1, purified immunoglobulin, buffered aqueous solution

• Synonim(y): Anti-ddddk, Anti-dykddddk
• UNSPSC Code: 12352203
• NACRES: NA.32
• MDL number: MFCD00212941
• Conjugate: unconjugated
• Clone: M1, monoclonal
• Application: FLAG purification, WB
• Citations: 139

Właściwości

• biological source: (Bioreactor)
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: M1, monoclonal
• form: buffered aqueous solution
• purified by: using Protein A
• species reactivity: all
• concentration: 2-5 mg/mL
• technique(s): FLAG purification: suitable, western blot: 10 μg/mL
• immunogen sequence: DYKDDDDK
• shipped in: dry ice
• storage temp.: −20°C

Opis

General description

The ANTI-FLAG M1 IgG2b mooclonal antibody binds to proteins with a FLAG marker at the free N-terminus.

Method of purification - Protein A

Immunogen

FLAG; peptide sequence DYKDDDDK

Application

Monoclonal ANTI-FLAG^® M1 antibody is suitable for immunoprecipitation, western blotting, or EIA in yeast, animal and E.coli cells.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.


Chromatin immunoprecipitation (1 paper)


Immunofluorescence (1 paper)

Learn more product details in our FLAG^® applications portal.

Biochem/physiol Actions

Binds to the FLAG epitope when it is located at the free amino-terminus of a fusion protein. Does not bind to Met-FLAG fusion proteins, so will not recognize unprocessed, cytoplasmically expressed proteins. Binding is Ca²⁺-dependent; the complex dissociates in the absence of calcium ions.

Physical form

Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v).

Preparation Note

Dilute antibody to 10ug/mL in .05 M TBS, pH 7.4, containing 1 mM CaCl2.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Klasa składowania: 12 - Non Combustible Liquids
• Co się dzieje?: WGK 2