This product is tested for suitability in insect cell culture. While not specifically tested on S2 cells, the product is expected to be suitable.
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F4135
Fetal Bovine Serum
USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma
Synonim(y):
HI FBS, HI FCS, HI sera, HI serum, FBS, FCS, sera, serum
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
100 mL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 860,00 zł |
500 mL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 3970,00 zł |
Informacje o tej pozycji
Biological source:
bovine fetus
Origin:
USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
860,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócbiological source
bovine fetus
Quality Segment
sterility
sterile-filtered
product line
FBS Classic
composition
Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL
origin
USA origin
technique(s)
cell culture | hybridoma: suitable, cell culture | insect: suitable, cell culture | mammalian: suitable
impurities
≤10 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
General description
Fetal Bovine Serum (FBS) remains a popular media supplement as it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and aids in proliferation. It also protects cells from oxidative damage and apoptosis. FBS blocks reactive oxygen species generation, which otherwise is not eliminated in serum-free media (SFM) systems.
Application
FBS sourced in the United States is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.
Fetal Bovine Serum has been used:
Fetal Bovine Serum has been used:
- as a component of Dulbecco′s Modified Eagles Media (DME, DMEM), Eagle′s Minimum Essential Medium (EMEM), Roswell Park Memorial Institute medium (RPMI-1640), α-modified eagle medium (αMEM) (MEM) and Leibovitz L-15 medium
- as a component of blocking medium for treating brainstem slices
- to improve attachment of dermal explants
Preparation Note
Prepared by heating at 56°C for 30 minutes.
This product is prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes inactivates the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.
Analysis Note
- Endotoxin and hemoglobin tested
- Tested for the presence of bacteria, virus, and mycoplasma
- Triple filtered with 0.1 micron membrane under aseptic conditions
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Ta pozycja | |||
|---|---|---|---|
| description USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma | description non-USA origin, γ-irradiated, sterile-filtered, suitable for cell culture | description USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma | description Heat Inactivated, non-USA origin, sterile-filtered, suitable for cell culture |
| technique(s) cell culture | insect: suitable, cell culture | hybridoma: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, cell culture | hybridoma: suitable | technique(s) cell culture | mammalian: suitable |
| origin USA origin | origin non-USA origin | origin USA origin | origin non-USA origin |
| sterility sterile-filtered | sterility γ-irradiated, sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| biological source bovine fetus | biological source bovine fetus | biological source bovine fetus | biological source bovine fetus |
| composition Hemoglobin, ≤20 mg/dL , Bovine IgG, ≤1 mg/mL | composition hemoglobin, ≤25 mg/dL | composition Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL | composition Hemoglobin, ≤25 mg/dL |
| impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin |
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Klasa składowania
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Is this FBS suitable for culturing fly S2 cells?
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Is the FBS F4135 tetracycline negative?
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This product is tetracycline negative.
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Is it possible to store the FBS at a temperature of -80°C, which is lower than the recommended storage temperature of -20°C?
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The FBS should be stored at the recommended temperature of -20°C. Storage at -80°C has not been tested, but there are no anticipated issues. Frost Free Freezers should be avoided for storage.
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Recommended storage is listed as -20C; is it safe/ok to store at -80C?
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The recommended long-term storage temperature for FBS is -20C. For the short-term, such as for shipping, packing on dry ice at -40C is recommended. It is not recommended to store the FBS at temperatures lower than -40C. Please see the guide on freezing and thawing FBS and other sera below:
https://www.sigmaaldrich.com/technical-documents/protocol/cell-culture-and-cell-culture-analysis/cell-culture-media-preparation/storing-and-thawing-serumHelpful?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Why is serum Heat-Inactivated (HI)?
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Heat inactivation of serum is performed to inactivate complement (which can cause cell lysis or downstream problems in immunoassays). If there are immunoglobulins present in the serum that can bind to the cells in culture (either specifically or non-specifically), there is a chance for antibody-mediated complement cytotoxicity (ADCC, causing cell death). It may also reduce the titer of some mycoplasma and some viruses. Often, heat-inactivated serum is used because of the adoption of a previous protocol. It is mainly used when culturing hematopoetic cells or performing immunological type assays such as mixed-lymphocyte reactions or neutralization assays.FBS may not be heat inactivated as this is not required for all applications. It limits the loss of the other protein activity that may be minimally present in the serum.A protocol for heat inactivation:1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.
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Does Sigma-Aldrich offer delipidated bovine serum?
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At this time, Sigma-Aldrich does not offer delipidated bovine serum (either FBS or calf serum).A method for lipid depletion, though not necessarily that of Sigma:1. While mixing, gradually add 10 g fumed silica (Product S5130 or Aerosil) per liter of serum2. Mix at room temperature and then transfer to 2-8 °C for overnight mixing.3. After overnight mixing, remove flumed silica (Aerosil) by centrifugation at 3000-5000 rpm for 5 minutes.4. Retain the supernatant. The lipids should stick to the silica. There may be some protein loss
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Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?
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Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored refrigerated at 2-8°C. If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days. For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance. For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C. For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.
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