Catalog number F7524 is not tested for tetracycline, so we cannot guarantee that the product is tetracycline free. Product F2442 is regularly tested for tetracycline and the results shown in a lot-specific certificate of analysis. The website page for this product is linked here: https://www.sigmaaldrich.com/catalog/product/sigma/f2442?lang=fr®ion=CH
F7524
Fetal Bovine Serum
non-USA origin, sterile-filtered, suitable for cell culture
Fetal Bovine Serum
1 of 8 reviewers received a sample product or took part in a promotion
Synonim(y):
Fetal Calf sera, Fetal Calf serum, FBS, FCS, sera, serum
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Wybierz wielkość
50 ML
375,00 zł
100 ML
629,00 zł
500 ML
3080,00 zł
375,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Informacje o tej pozycji
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Pomoc techniczna
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Pozwól nam pomócpochodzenie biologiczne
bovine fetus
Poziom jakości
sterylność
sterile-filtered
linia produktu
FBS Classic
skład
hemoglobin, ≤25 mg/dL
pochodzenie
non-USA origin
dostępność
not available in Canada, USA
metody
cell culture | mammalian: suitable
zanieczyszczenia
≤10 EU/mL endotoxin
Warunki transportu
dry ice
temp. przechowywania
−20°C
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Ta pozycja | 12003C | F6765 | F0392 |
|---|---|---|---|
| origin non-USA origin | origin Australia origin | origin USA origin | origin USA origin |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| biological source bovine fetus | biological source bovine fetus | biological source bovine fetus | biological source bovine fetus |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| composition hemoglobin, ≤25 mg/dL | composition Hemoglobin, ≤25 mg/dL | composition Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL | composition Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Zastosowanie
Fetal Bovine Serum has been used as a supplement in cell culture media such as Roswell Park Memorial Institute medium (RPMI-1640) and Dulbecco′s Modified Eagles Media (DMEM)[1]to support the growth of a variety of cell lines.
Działania biochem./fizjol.
Fetal bovine serum (FBS) serves as a commonly utilized cell culture media supplement to support in vitro and ex vivo cell, tissue, and organ cultures. FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth, and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems. It provides many non-defined growth-promoting and survival enhancing factors to cells in culture. Derived from clotted blood, it is the most widely used undefined supplement in eucaryotic, especially mammalian, cell culture. FBS is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640.
Cechy i korzyści
F7524 is the same great product as Biochrom GmbH. product S0115.
Komentarz do analizy
- Endotoxin and hemoglobin tested
- Tested for the presence of bacteria, virus, and mycoplasma
- Triple filtered with 0.1 micron membrane under aseptic conditions
Informacje prawne
Not available in USA or Canada. Please check with your local supplier regarding availability and import restrictions in your country.
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produkt powiązany
Numer produktu
Opis
Cennik
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Elisabeth F P Peterse et al.
BMC cancer, 16, 475-475 (2016-07-16)
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Hans F N Kvitvang et al.
Journal of chromatography. A, 1370, 70-79 (2014-12-03)
Metabolic profiling has become an important tool in biological research, and the chromatographic separation of metabolites coupled with mass spectrometric detection is the most frequently used approach for such studies. The establishment of robust chromatographic methods for comprehensive coverage of
Carlo E A Jochems et al.
Alternatives to laboratory animals : ATLA, 30(2), 219-227 (2002-04-25)
Fetal bovine serum (FBS) is a common component of animal cell culture media. It is harvested from bovine fetuses taken from pregnant cows during slaughter. FBS is commonly harvested by means of a cardiac puncture without any form of anaesthesia.
Jiří Voller et al.
Bioorganic chemistry, 90, 103005-103005 (2019-07-05)
Rho-associated serine/threonine kinases (ROCKs) are principal regulators of the actin cytoskeleton that regulate the contractility, shape, motility, and invasion of cells. We explored the relationships between structure and anti-ROCK2 activity in a group of purine derivatives substituted at the C6
Michelle J Nyhan et al.
BMC cancer, 16, 101-101 (2016-02-18)
Successful treatment of oesophageal cancer is hampered by recurrent drug resistant disease. We have previously demonstrated the importance of apoptosis and autophagy for the recovery of oesophageal cancer cells following drug treatment. When apoptosis (with autophagy) is induced, these cells
Produkty
Zapoznaj się z podstawami stosowania płodowej surowicy bydlęcej (lub cielęcej) do hodowli komórek oraz z tym, jak pozyskiwanie i przepisy wpływają na cenę surowic do testów komórkowych.
Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.
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Does the F7524 Fetal Bovine Serum product contain Tetracycline?
1 answer-
Helpful?
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When aliquioting FBS, is it best to cool them to 4ºC first or directly at -80ºC
1 answer-
The serum can be aliquoted into smaller, single-use portions and refrozen directly.
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Hello, I have a question regarding FBS (F7524), for my experiment it is essential there is no dopamine or serotonine in my medium. Is it known wether these substances are present in FBS?
1 answer-
This product has not undergone testing to determine the presence of dopamine or serotonin.
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Could the presence of insoluble precipitates when thawing the serum indicate contamination?
1 answer-
Thawed serum may contain precipitates and flocculants due to various reasons, such as residual fibrinogen converting to fibrin upon thawing. Centrifugation at 400 xg can remove excess fibrin, while filtering serum is not recommended to avoid protein depletion and clogged filters.
Additional points to consider:
•Cloudiness in human serum products is common and indicates the presence of lipids. Clear, lipid-free human serum products are not available.
•Microscopic serum precipitates may resemble yeast contamination but are actually lipid aggregates that can be dissolved by lipase. They do not show growth in culture or gram staining and can be removed through centrifugation or filtration.
•Gel layer or clumps in serum are caused by poor or overheated inactivation or improper mixing of FBS during the Heat inactivation process. Serum with these characteristics should not be used.
•To minimize denaturation of serum lipoproteins and turbidity, it is recommended to prepare single-use aliquots and avoid repeated freeze-thaw cycles.Helpful?
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Is this product F7524 heat-inactivated? Should it be prior to use?
1 answer-
This product has not been heat inactivated. For most cell culture applications heat inactivation is not necessary. If the application requires inactivation of any heat labile serum components, such as compliment or cytokines, the product may be heat inactivated in the lab. Alternatively, a heat inactivated version of this product is also available. See product F9665.
To heat inactivate this product:
1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.
2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.
3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.
4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Avoid repeated thaw-freeze cycles.Helpful?
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Bonjour, Quelle est la composition et la concentration du sérum référencé F7524 en Calcium ? Cordialement
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Calcium levels are not determined for this product. The serum is a natural product that will contain calcium at physiological levels.
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Welches fetale Kälbserum wurde für die Kryokonservierung von Mausembryonen oder Eiestockmäus verwendet?
1 answer-
Unfortunately, a fetal calf serum that has been specifically tested for cryopreservation of mouse embryos or ovarian mice is not currently offered. Please see the link below to review Product ES009-M, Fetal Bovine Serum. This product is tested for expansion and cytotoxicity of mouse embryonic stem cells and may be of interest:
https://www.sigmaaldrich.com/product/mm/es009mHelpful?
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How can we heat inactivate the fetal bovine serum cat: F7524? For how long?
1 answer-
All heat inactivated serum products are treated at 56 deg. C for 30 minutes. This process may be applied to manually heat inactivate serum on a per bottle basis using a water bath. When immersing bottles do not allow water to come in contact with the cap/closure.
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Is Feta bovine serum F7524-500ML heat inactivated?
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This product is not heat-inactivated.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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