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Anti-Mouse IgG (Fab specific) F(ab′)2 fragment–FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Numer MDL:

Poziom jakości


pochodzenie biologiczne


białko sprzężone

FITC conjugate

forma przeciwciała

affinity isolated antibody

antibody product type

secondary antibodies




buffered aqueous solution

warunki przechowywania

protect from light


immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320

temp. przechowywania


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Opis ogólny

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.
Immunoglobulin G (IgG) is a member of the immunoglobulin family and contains gamma (γ) heavy chain in the constant (C) region. Monomeric structure of IgG contains two identical heavy chains and two identical light chains with molecular weight of 50 kDa and 25 kDa respectively. The primary structure of this antibody also contains disulfide bonds implicated in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3 and IgG4 with different heavy chains, named γ1, γ2, γ3 and γ4, respectively.


Binds all mouse Igs.
Useful when trying to avoid background staining due to the presence of Fc receptors. Anti-Mouse IgG (Fab specific) F(ab′)2 fragment-FITC antibody recognizes Fab fragment of mouse IgG. No cross reaction is observed with the Fc fragment of mouse IgG. No reactivity is observed with human, horse or bovine serum. The antibody is purified by affinity isolation and conjugated with FITC in an alkaline reaction.


Purified mouse IgG; The F(ab′)2 fragment of the antibody is obtained from pepsin digested antiserum


Anti-Mouse IgG (Fab specific) F(ab′)2 fragment−FITC antibody produced in goat has been used in:
  • flow cytometry for fluorescence-activated cell sorting (FACS) analysis
  • immunofluorescence and confocal microscopy
  • immunofluorescent HSP72 detection/immunocytochemistry experiments

Działania biochem./fizjol.

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG2a has cytotropic properties and IgG2 molecules have the ability to activate the complement system.
Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.

Inne uwagi

Antibody adsorbed with bovine, equine and human serum proteins.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Uwaga dotycząca przygotowania

Adsorbed to reduce background staining with bovine, horse or human samples.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Kod klasy składowania

12 - Non Combustible Liquids



Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certyfikat analizy

Wprowadź numer partii, aby wyszukać certyfikat analizy (COA).

Świadectwo pochodzenia

Wprowadź numer partii, aby wyszukać świadectwo pochodzenia (COO).

Quotes and Ordering

Recombinant adenoviruses expressing dominant negative insulin-like growth factor-I receptor demonstrate antitumor effects on lung cancer
Lee CT, et al.
Cancer Gene Therapy, 10(1), 57-57 (2003)
Adela Quesada et al.
Journal of neuroscience research, 89(5), 674-688 (2011-02-22)
The retina of nonmammalian vertebrates has a loose myelin that enwraps the large axons of the ganglion cells in all areas, whereas that of mammals lacks myelin, with some exceptions, such as the rabbit retina, which shows compact myelin restricted
The Immunoglobulins: Structure and Function (1998)
Kyeng Gea Lee et al.
Cell motility and the cytoskeleton, 64(8), 621-628 (2007-05-18)
One prominent cytoskeletal feature of non-mammalian vertebrate erythrocytes is the marginal band (MB), composed of microtubules. However, there have been several reports of MB-associated F-actin. We have further investigated the function of MB-associated F-actin, using newt erythrocytes having large, thick
Foad J Rouhani et al.
Transfusion, 44(7), 1004-1012 (2004-07-01)
Pigs are a potential source of red blood cells (RBCs) for transfusion into humans, but the presence of galactose-alpha1,3-galactose (Gal) epitopes on their surface, against which humans have anti-Gal, has been perceived as a major barrier. alpha1,3-Galactosyltransferase gene-knockout pigs, which

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