G4252

Glyoxalase I from Saccharomyces cerevisiae

Grade IV, buffered aqueous glycerol solution, ≥400 units/mg protein

• Synonim(y): S-Lactoyl-glutathione methylglyoxal-lyase (isomerizing)
• Numer CAS: 9033-12-9
• UNSPSC Code: 12352204
• NACRES: NA.54
• Numer WE: 4.4.1.5 (BRENDA, IUBMB)
• MDL number: MFCD00131225
• Specific activity: ≥400 units/mg protein

Właściwości

• type: Grade IV
• Quality Segment: 200
• form: buffered aqueous glycerol solution
• specific activity: ≥400 units/mg protein
• foreign activity: glyoxalase II ≤1%
• storage temp.: 2-8°C

Opis

General description

Glyoxalase detoxification system consists of glyoxalase (GLO)-I and GLO-II. GLO-I is a cytosolic, 42 kDa, dimeric Zn²⁺ metalloenzyme.

Application

Glyoxalase I from Saccharomyces cerevisiae has been used as a standard in Glyoxalase I assay for C. dilatata females and brooded encapsulated embryos. It has also been used as a standard for calibration curve generation for quantifying glyoxalase I from brain tissues and cerebral microvessels.

Biochem/physiol Actions

Glyoxalase I is universally expressed and involved in the protection against cellular damage due to cytotoxic metabolites such as advanced glycation end products (AGEs). It is an integral component of the detoxification system, catalyzing the conversion of reactive, acyclic a-oxoaldehydes into the corresponding a-hydroxyacids in a glutathione-dependent manner.

Physical form

Solution in 50% glycerol, 0.4 M (NH₄)₂SO₄ and 0.002 M KH₂PO₄ pH 6.5

Analysis Note

Protein determined by biuret.

Other Notes

One unit will form 1.0 μmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per min at pH 6.6 at 25 °C.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 10 - Combustible liquids
• Co się dzieje?: WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• PPE (środki ochrony indywidualnej): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)