G5003

α-Glucosidase from Saccharomyces cerevisiae

Type I, lyophilized powder, ≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.)

• Synonim(y): α-D-Glucosidase, α-D-Glucoside glucohydrolase, Maltase from yeast
• Numer CAS: 9001-42-7
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-604-7
• MDL number: MFCD00081321
• Numer WE: 3.2.1.20 (BRENDA, IUBMB)
• Specific activity: ≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.)
• Assay: ≥50% (biuret)
• Biological source: Saccharomyces cerevisiae

Właściwości

• Quality Segment: 200
• biological source: Saccharomyces cerevisiae
• type: Type I
• assay: ≥50% (biuret)
• form: lyophilized powder
• specific activity: ≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.)
• contains: phosphate buffer salts and EDTA as balance
• composition: Protein, ≥50%
• sustainability: Sustainability features
• optimum pH: 6.8
• UniProt accession no.: P38158, P53341
• foreign activity: β-Glucosidase, α−galactosidase and β−galactosidase ≤0.1%
• relevant disease(s): diabetes (management of non-insulin-dependent diabetes mellitus)
• storage temp.: −20°C
• Gene Information: bakers yeast ... MAL12(853209), MAL32(852602)

Opis

General description

Substrate Specificity

Hydrolysis of terminal, non-reducing (1→4)-linked α-D-glucose residues with release of α-D-glucose

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch hydrolysis research. For more information see the article in biofiles.

Application

α-glucosidase is used for the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters. It was also used in the measurement of glycosidase inhibition.

For the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters

Biochem/physiol Actions

α-glucosidase hydrolyzes carbohydrates by acting on 1,4-α linkages. Inhibition of α-glucosidase is a prominent target in the management of non-insulin-dependent diabetes mellitus.

Hydrolysis of terminal, non-reducing 1→4-linked D-glucose residues with release of D-glucose.

Packaging

Sold on basis of p-nitrophenyl α-D-glucoside units.

Analysis Note

Protein determined by biuret.

Other Notes

One unit will liberate 1.0 μmole of D-glucose from p-nitrophenyl α-D-glucoside per min at pH 6.8 at 37 °C.

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• WGK: WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• PPE (środki ochrony indywidualnej): Eyeshields, Gloves, type N95 (US)