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H8032
Hanahan′s Broth (SOB Medium)
powder microbial growth medium
Synonim(y):
Super Optimal Broth
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
500 g | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 818,00 zł |
Informacje o tej pozycji
NACRES:
NA.85
UNSPSC Code:
41106200
818,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Molecular Biology
Quality Segment
description
recommended for cultivation of recombinant E. coli.
sterility
non-sterile
form
powder
composition
KCl, 0.186 g/L , MgSO4, 2.4 g/L , NaCl, 0.5 g/L , Tryptone, 20 g/L , Yeast extract, 5 g/L
technique(s)
microbiological culture: suitable, molecular cloning: suitable
pH
6.8-7.2(2.8% solution)
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
Hannahan’s Broth (SOB) is a microbial growth medium used for the preparation and transformation of competent cells (E. coli). Casein enzymic hydrolysate and yeast extract supply nitrogenous compounds and growth factors for recombinant Escherichia coli. Potassium chloride and sodium chloride maintain the isotonic condition. Magnesium sulfate is added to the medium as a necessary component for DNA replication.
Application
Hanahan′s Broth is recommended for use in cultivation of recombinant strains of Escherichia coli. It is suitable for the preparation of competent E. coli., often with the addition of 0.4% dextrose. It has also been used for the preparation of C. necator H16 competent cells. It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
Features and Benefits
Hannahan′s Broth (SOB) powder provides:
- Standard formulation
- An inexpensive alternative to liquid
Preparation Note
Stir to suspend 28 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Other Notes
Casein enzymic hydrolysate and yeast extract supply the necessary nitrogenous compounds and growth factors. Potassium chloride and sodium chloride provide an isotonic environment for the cells. Magnesium sulfate aids DNA replication.
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Ta pozycja | |||
|---|---|---|---|
| description powder microbial growth medium | description For use in transformation | description Highly-referenced nutrient-rich microbial growth powder medium, suitable for regular E.coli culture | description Powder microbial growth medium |
| technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) - | technique(s) microbiological culture: suitable |
| form powder | form liquid | form powder | form powder |
| suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for coliforms, nonselective for Escherichia coli | suitability nonselective for coliforms, nonselective for Escherichia coli |
| sterility non-sterile | sterility 0.2 μm filtered | sterility non-sterile | sterility non-sterile |
| composition MgSO4, 2.4 g/L , Tryptone, 20 g/L , Yeast extract, 5 g/L , NaCl, 0.5 g/L , KCl, 0.186 g/L | composition Dextrose, 3.603 g/L , KCl, 0.186 g/L , MgSO4, 4.8 g/L , Tryptone, 20 g/L , Yeast extract, 5 g/L | composition Tryptone, 10 g/L , Yeast Extract, 5 g/L , NaCl, 10 g/L | composition Casamino Acids, 1 g/L , Yeast extract, 5 g/L , Tryptone, 10 g/L , NaCL, 5 g/L , MgSO4, 0.98 g/L |
| application(s) food and beverages | application(s) agriculture | application(s) food and beverages | application(s) food and beverages |
Klasa składowania
10 - Combustible liquids
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
PPE (środki ochrony indywidualnej)
Eyeshields, Gloves
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What is the Department of Transportation shipping information for this product?
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Helpful?
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Which bacterial culture medium is the best choice for my application?
1 answer-
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
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