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K4002

Krebs-Ringer Bicarbonate Buffer

With 1800 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

Synonim(y):

Bicarbonate Buffer Solution, Krebs Buffer, Krebs-Ringer Solution

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Rozmiar/SKUDostępnośćCena netto
10 x 1 L
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1070,00 zł

Informacje o tej pozycji

NACRES:
NA.75
UNSPSC Code:
12161702

1070,00 zł


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Quality Segment

form

powder

technique(s)

cell culture | mammalian: suitable

pH

6.1-6.7

solubility

water: 9.5 g/L, clear

storage temp.

2-8°C

General description

Krebs-Ringer Bicarbonate Buffer acts as a salt solution and plays an essential role in tissue culture. A salt solution provides the cells with water and inorganic ions. It also helps in maintaining the osmotic balance and ideal pH in the medium. This bicarbonate buffered isotonic saline contains magnesium, potassium, sodium, and phosphate ions.

Application

Krebs-Ringer Bicarbonate Buffer has been used:

  • as a wash buffer for the preparation of mice primary adipocytes and lipolysis assay
  • in the pre-perifusion for islet viability and functionality studies
  • in pituitary bioassay

Preparation Note

Formulated to contain 9.5 grams of powder per liter of medium.
Supplement with 1.26 g/L sodium bicarbonate.
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Ta pozycja
T2397C3041H6136
description

With 1800 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

description

With sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

description

capsule

description

Without sodium bicarbonate, powder, suitable for cell culture

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

Quality Level

500

Quality Level

400

Quality Level

200

Quality Level

500

form

powder

form

liquid

form

capsule

form

powder

storage temp.

2-8°C

storage temp.

room temp

storage temp.

-

storage temp.

2-8°C

pH

6.1-6.7

pH

-

pH

9.3-9.9

pH

-

solubility

water: 9.5 g/L, clear

solubility

-

solubility

water, high purity: 1 capsule/100 mL

solubility

-


Klasa składowania

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

1–5 of 5 Questions  
  1. Do you have a glucose free product?

    1 answer
    1. Unfortunately, a Krebs-Ringer Bicarbonate Buffer without glucose is not available.

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  2. Product K4002 - Krebs-Ringer Bicarbonate Buffer, is without calcium chloride and sodium bicarbonate. In the reconstitution steps, you advice to add 1.26g sodium bicarbonate. Should we also add some calcium chloride? in which amount?

    1 answer
    1. Product K4002 - Krebs-Ringer Bicarbonate Buffer does not contain calcium. The addition of calcium is not required for osmolarity.  If you need to have calcium, you can add calcium chloride between 1.25 to 2.5 mM.  You may want to check the osmolarity and make sure it is not too high.Kreb Henseleit Buffer has 1.25 mM calcium chlorideKreb's Buffer has 2.5 mM calcium chlorideKreb' Ringer Superfusion solution contains 2.0 mM calcium chloride.Please see the attached document for different Krebs' buffer recipes.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. What is the formula of Product K4002, Krebs-Ringer Bicarbonate Buffer (KRB)?

    1 answer
    1. The formulation for KRB can be found on the data sheet.

      Helpful?

  5. How should Product K4002, Krebs-Ringer Bicarbonate Buffer, be dissolved?

    1 answer
    1. Product No. K4002 is listed in a 10X1L package size, meaning that each of the ten containers has enough powder to make 1 Liter of KRB buffer. It can be dissolved in 1 Liter of either water or medium.

      Helpful?

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