LB Broth with agar (Luria low salt) (L3272) is generally suitable for microbiological culture and is nonselective, but there is no explicit confirmation in the provided documentation that it is suitable for Bacillus megaterium.
Przejdź do
L3272
Bulion LB z agarem (o niskiej zawartości soli Luria)
Powder microbial growth medium
Synonim(y):
Luria Agar Base (Miller’s Modification)
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
1 kg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1300,00 zł |
Informacje o tej pozycji
NACRES:
NA.85
UNSPSC Code:
41106200
1300,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócgrade
Molecular Biology
Quality Segment
sterility
non-sterile
form
powder
composition
Agar, 15 g/L , NaCl, 0.5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L
technique(s)
microbiological culture: suitable
pH
6.8-7.2(3.05% solution)
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
Luria's low salt LB to podłoże do wzrostu drobnoustrojów stosowane do hodowli E. coli. Ten bogaty w składniki odżywcze bulion mikrobiologiczny zawiera peptydy, aminokwasy, rozpuszczalne w wodzie witaminy i węglowodany. Dodatek agaru zapewnia stałe podłoże do wzrostu drobnoustrojów.
Application
Użyto bulionu LB z agarem (Luria low salt):
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.
Features and Benefits
Proszek Luria o niskiej zawartości soli z agarem zapewnia:
- Łatwe skalowanie przy użyciu większych opakowań
- Przyjazna dla budżetu alternatywa dla wstępnie wylanych płytek
- Standardowy skład
Preparation Note
1. Zawiesić 30,5 g w 1 l wody destylowanej.
2. Podgrzać do wrzenia, mieszając do rozpuszczenia.
3. Autoklawować przez 15 minut w temperaturze 121°C.
4. Schłodzić do 50°C przed dozowaniem do szalek Petriego.
2. Podgrzać do wrzenia, mieszając do rozpuszczenia.
3. Autoklawować przez 15 minut w temperaturze 121°C.
4. Schłodzić do 50°C przed dozowaniem do szalek Petriego.
Legal Information
Zeocin is a registered trademark of Cayla Sarl
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Ta pozycja | |||
|---|---|---|---|
| description Powder microbial growth medium | description Highly-referenced nutrient-rich microbial growth powder medium with Agar, suitable for regular E.coli culture. | description Powder microbial growth medium | description Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture. |
| form powder | form powder | form powder | form powder |
| suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability suitable for molecular biology, nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms |
| technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable |
| composition NaCl, 0.5 g/L , Tryptone, 10 g/L , Agar, 15 g/L , Yeast Extract, 5 g/L | composition Tryptone, 10 g/L , Agar, 15 g/L , Yeast Extract, 5 g/L , NaCl, 10 g/L | composition Yeast Extract, 5 g/L , Tryptone, 10 g/L , NaCl, 0.5 g/L | composition Tryptone, 10 g/L , Yeast Extract, 5 g/L , NaCl, 5 g/L , Agar, 15 g/L |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| application(s) food and beverages | application(s) food and beverages | application(s) food and beverages | application(s) food and beverages |
Klasa składowania
11 - Combustible Solids
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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Is it suitable for b. Megaterium culture ?
1 answer-
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What is the difference between the LB - Luria low salt products?
1 answer-
LB - Luria low salt is available in both powder (L3397) and powder with agar formats (L3272).
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Will adding magnesium to the culture medium increase cell density?
1 answer-
In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
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What are the differences among the Luria, Lennox and Miller LB formulations?
1 answer-
LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
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Which bacterial culture medium is the best choice for my application?
1 answer-
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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