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M0268

Minimum Essential Medium Eagle

With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonim(y):

EMEM, MEM

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Informacje o tej pozycji

NACRES:
NA.75
UNSPSC Code:
12352207
Informacje o cenach i dostępności nie są obecnie dostępne.
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form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
L-glutamine: yes
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

General description

Listen to an Audio Overview
Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. MEM has been used for culturing several cells grown in monolayers. It is commonly used to grow attached cell lines, such as fibroblasts, in the presence of fetal bovine sera (FBS), calf, or horse sera.

Application

Minimum Essential Medium Eagle has been used:
  • in the isolation and culture of primary hepatocytes
  • to culture the human endometrial carcinoma cells Ishikawa (ATTC) and L-Wnt-3a cells (ATCC) that secrete Wnt family member 3a (Wnt-3a) ligand
  • to culture the AN3CA (endometrial cancer cell line) cells, and HEC1-B human (endometrial adenocarcinoma cell line) cells

Features and Benefits

  • Minimum Essential Medium Eagle (MEM) contains higher concentrations of amino acids to mimic the protein composition of cultured mammalian cells.
  • The formulation has been altered by optional elimination of calcium to enable the growth of cells in suspension culture.

Preparation Note

Formulated to contain 9.5 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.

Other Notes

Essentially the same as the original formula, except it is provided as a powder.
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Ta pozycja
M0643M4642M0275
Minimum Essential Medium Eagle With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sigma-Aldrich

M0268

Minimum Essential Medium Eagle

Minimum Essential Medium Eagle With Earle′s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

Sigma-Aldrich

M0643

Minimum Essential Medium Eagle

Minimum Essential Medium Eagle With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sigma-Aldrich

M4642

Minimum Essential Medium Eagle

Minimum Essential Medium Eagle 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M0275

Minimum Essential Medium Eagle

description

With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

description

With Earle′s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

description

With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

description

10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

form

powder

form

powder

form

powder

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
L-glutamine: yes
sodium pyruvate: no

components

HEPES: no
Earle’s salts (5% CO2): yes
NaHCO3: no
phenol red: yes
L-glutamine: yes
sodium pyruvate: no

components

glucose: yes
Hanks’ salts (2% CO2): yes
L-glutamine: yes
Earle’s salts (5% CO2): no
NaHCO3: no
phenol red: yes
sodium pyruvate: no
stable glutamine: no
HEPES: no

components

phenol red: yes
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no
sodium pyruvate: no
NaHCO3: no

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

classic media, classical media, FBS

classic media, classical media, FBS

EMEM for tissue culture applications, Minimum Essential Medium Eagle 1L, SAFC EMEM for mammalian cell growth, MEM, classic media, classical media, FBS, high concentration amino acids MEM for cell culture, 500mL MEM Eagle for scientific research, EMEM cell culture media, Minimum Essential Medium Eagle with non-essential amino acids, cell suspension culture media MEM, SAFC Minimum Essential Medium Eagle 500mL, cell culture media MEM without calcium, Mammalian cell culture MEM 500mL, Minimum Essential Medium Eagle formulation with Hanks′ salts, MEM Eagle formulation for mammalian cells, Minimum Essential Medium Eagle for lab

MEM, classic media, classical media, FBS

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Klasa składowania

13 - Non Combustible Solids

Co się dzieje?

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Wykazy regulacyjne

Wykazy regulacyjne dotyczą głównie produktów chemicznych. Można w nich podawać ograniczoną liczbę informacji na temat produktów niechemicznych. Brak wpisu oznacza, że żaden ze składników nie znajduje się w wykazie. Użytkownik odpowiada za zagwarantowanie bezpiecznego i zgodnego z prawem stosowania produktu.

10035-04-8

CAS No.

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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–5 of 5 Questions  

  1. Does Product M0268, Minimum Essential Medium Eagle, have an expiration date?

    1 answer

    1. Yes, this product has an expiration date of 36 months from the date of manufacture. The expiration date can be found on the Certificate of Analysis.

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  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  3. Does Product M0268, Minimum Essential Medium Eagle, contain phenol red?

    1 answer

    1. Yes, this product contains phenol red.  Phenol red is included in most medium as a visual pH indicator.  The medium should be changed when the color turns yellow (acidic) or purple (basic).

      Helpful?

  4. Does Product M0268, Minimum Essential Medium Eagle, contain sodium bicarbonate?

    1 answer

    1. No, this product does not contain sodium bicarbonate. It is recommended to add 2.2 g sodium bicarbonate per Liter of medium. Use either Product No. S5761 (powder) or S8761 (7.5% solution).

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  5. What is the formulation of Product M0268, Minimum Essential Medium Eagle?

    1 answer

    1. The formulation for this product can be found on the product datasheet.

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