No, this product does not contain sodium bicarbonate. It is recommended to add 2.2 g sodium bicarbonate per Liter of medium. Use either Product No. S5761 (powder) or S8761 (7.5% solution).
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M0268
Minimum Essential Medium Eagle
With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Synonim(y):
EMEM, MEM
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| Rozmiar/SKU | Dostępność | Cena netto |
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Informacje o tej pozycji
NACRES:
NA.75
UNSPSC Code:
12352207
eCl@ss:
32160701
Informacje o cenach i dostępności nie są obecnie dostępne.
Pomoc techniczna
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Pozwól nam pomócform
powder
technique(s)
cell culture | mammalian: suitable
components
HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
L-glutamine: yes
sodium pyruvate: no
shipped in
ambient
storage temp.
2-8°C
General description
Listen to an Audio Overview
Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. MEM has been used for culturing several cells grown in monolayers. It is commonly used to grow attached cell lines, such as fibroblasts, in the presence of fetal bovine sera (FBS), calf, or horse sera.
Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. MEM has been used for culturing several cells grown in monolayers. It is commonly used to grow attached cell lines, such as fibroblasts, in the presence of fetal bovine sera (FBS), calf, or horse sera.
Application
Minimum Essential Medium Eagle has been used:
- in the isolation and culture of primary hepatocytes
- to culture the human endometrial carcinoma cells Ishikawa (ATTC) and L-Wnt-3a cells (ATCC) that secrete Wnt family member 3a (Wnt-3a) ligand
- to culture the AN3CA (endometrial cancer cell line) cells, and HEC1-B human (endometrial adenocarcinoma cell line) cells
Features and Benefits
- Minimum Essential Medium Eagle (MEM) contains higher concentrations of amino acids to mimic the protein composition of cultured mammalian cells.
- The formulation has been altered by optional elimination of calcium to enable the growth of cells in suspension culture.
Preparation Note
Formulated to contain 9.5 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.
Other Notes
Essentially the same as the original formula, except it is provided as a powder.
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Ta pozycja | |||
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| description With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture | description With Earle′s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture | description With Hanks′ salts, L-glutamine and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture | description 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form powder | form powder | form powder | form liquid |
| components HEPES: no | components HEPES: no | components phenol red: yes | components phenol red: yes |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
classic media, classical media, FBS | classic media, classical media, FBS | MEM, classic media, classical media, FBS | MEM, classic media, classical media, FBS |
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Klasa składowania
13 - Non Combustible Solids
WGK
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Wykazy regulacyjne
Wykazy regulacyjne dotyczą głównie produktów chemicznych. Można w nich podawać ograniczoną liczbę informacji na temat produktów niechemicznych. Brak wpisu oznacza, że żaden ze składników nie znajduje się w wykazie. Użytkownik odpowiada za zagwarantowanie bezpiecznego i zgodnego z prawem stosowania produktu.
10035-04-8
CAS No.
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Does Product M0268, Minimum Essential Medium Eagle, contain sodium bicarbonate?
1 answer-
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Does Product M0268, Minimum Essential Medium Eagle, have an expiration date?
1 answer-
Yes, this product has an expiration date of 36 months from the date of manufacture. The expiration date can be found on the Certificate of Analysis.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the formulation of Product M0268, Minimum Essential Medium Eagle?
1 answer-
The formulation for this product can be found on the product datasheet.
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Does Product M0268, Minimum Essential Medium Eagle, contain phenol red?
1 answer-
Yes, this product contains phenol red. Phenol red is included in most medium as a visual pH indicator. The medium should be changed when the color turns yellow (acidic) or purple (basic).
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