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M3003
Myokinase from rabbit muscle
ammonium sulfate suspension, 1,500-3,000 units/mg protein (biuret)
Synonim(y):
ATP: AMP phosphotransferase, Adenylate Kinase
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| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 1000 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 331,00 zł | |
| 2500 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 616,00 zł | |
| 5000 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 959,00 zł | |
| 10000 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1680,00 zł |
Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-743-3
NACRES:
NA.54
MDL number:
Specific activity:
1,500-3,000 units/mg protein (biuret)
Biological source:
rabbit muscle
331,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócbiological source
rabbit muscle
Quality Level
form
ammonium sulfate suspension
specific activity
1,500-3,000 units/mg protein (biuret)
mol wt
21 kDa
foreign activity
3-phosphoglyceric phosphokinase ≤0.05%, lactic dehydrogenase and pyruvate kinase ≤0.01%
storage temp.
2-8°C
General description
Research area: Cell Signaling
Myokinase, also called Adenylate kinase or ADK, is a phosphotransferase enzyme. It belongs to the family of nucleoside monophosphate kinases. Myokinase is an enzyme that can be found in skeletal muscle and acts as a phosphotransferase agitator.
Myokinase, also called Adenylate kinase or ADK, is a phosphotransferase enzyme. It belongs to the family of nucleoside monophosphate kinases. Myokinase is an enzyme that can be found in skeletal muscle and acts as a phosphotransferase agitator.
Application
Myokinase has been used:
- in a study to assess its reaction with the mercurials p-hydroxymercuribenzoate and p-mercuribenzenesulfonate [1]
- in a study to investigate the ligand-binding properties of its peptide fragments [2]
- as one of the components in the ATP generation solution for the quantification of 3’3’-c-di-AMP using an AMP-Luciferase assay [3]
Biochem/physiol Actions
Myokinase or adenylate kinases, play a crucial role in regulating energy metabolism within cells and balancing adenine nucleotide ratios in different cellular compartments.
Myokinase is a key enzyme involved with adenine nucleotide metabolism. The action of myokinase insures that the adenine nucleotides (ATP, ADP, and AMP) are maintained in equilibrium in the cytosol.
Myokinase catalyzes the following reaction:ADP + ADP → ATP + AMP
Myokinase is a key enzyme involved with adenine nucleotide metabolism. The action of myokinase insures that the adenine nucleotides (ATP, ADP, and AMP) are maintained in equilibrium in the cytosol.
Myokinase catalyzes the following reaction:ADP + ADP → ATP + AMP
Rabbit muscle myokinase can be inactivated by treatment with dithiobis-(2-nitrobenzoic) acid which alters methionine residues critical to myokinase activity. [4]
Physical form
Suspension in 3.2 M (NH4)2SO4 solution containing 0.001 M EDTA, pH 6.0
Other Notes
One unit will convert 2.0 μmoles of ADP to ATP+AMP per min at pH 7.6 at 37°C.
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Ta pozycja | |||
|---|---|---|---|
| specific activity 1,500-3,000 units/mg protein (biuret) | specific activity 350-600 units/mg protein | specific activity 350-600 units/mg protein | specific activity ≥4,000 units/mg protein |
| biological source rabbit muscle | biological source rabbit muscle | biological source rabbit muscle | biological source rabbit muscle |
| form ammonium sulfate suspension | form ammonium sulfate suspension | form lyophilized powder | form ammonium sulfate suspension |
| mol wt 21 kDa | mol wt 237 kDa | mol wt 237 kDa | mol wt calculated mol wt 53.2 kDa |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Klasa składowania
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Produkty
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Powiązane treści
The sulfhydryl groups of rabbit muscle adenosine triphosphate-adenosine monophosphate phosphotransferase. Activity of enzyme treated with mercurials.
L F Kress et al.
The Journal of biological chemistry, 241(10), 2293-2300 (1966-05-25)
Likui Feng et al.
Nature communications, 12(1), 4912-4912 (2021-08-15)
Polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) hybrid systems typically use complex protein-protein interactions to facilitate direct transfer of intermediates between these multimodular megaenzymes. In the canal-associated neurons (CANs) of Caenorhabditis elegans, PKS-1 and NRPS-1 produce the nemamides, the only known
Secretion from dense granules: luminescence method for adenine nucleotides.
M Fred Heath
Methods in molecular biology (Clifton, N.J.), 272, 89-93 (2004-07-01)
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| M3003-1KU | 04061832924526 |
| M3003-2.5KU | 04061834046295 |
| M3003-10KU | 04061832924519 |
| M3003-5KU | 04061832924540 |