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M5550

MEM Amino Acids (50x) solution

Without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonim(y):

MEM Amino Acids mixture

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Informacje o tej pozycji

UNSPSC Code:
12352209
eCl@ss:
32160406
NACRES:
NA.75
MDL number:
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Quality Segment

sterility

sterile-filtered

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

Application

Adding supplements of amino acids to media stimulates growth and prolongs the viability of cells in culture. MEM Amino Acids (50x) solution concentrated supplement adds nutrients and reduces the biosynthetic burden on cell in vitro. It has been used in in vitro cultures, co-cultures and incubation media.

Preparation Note

This product may contain a yellow hue if exposed to light. This is due to the presence of the amino acids tryosine and tryptophan in the solution. The change in color to the liquid solution has no affect to the quality or integrity of the product. For a complete list of components, please see the formulation sheet listed in the "Documents" section of the product page.
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Ta pozycja
M7145M6895SCM081
description

Without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture

description

without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture

description

sterile-filtered, BioReagent, suitable for cell culture

description

The N21 Medium Supplement is an optimized serum-free supplement developed for isolation & expansion of neurons from mouse & rat hippocampus.

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | stem cell: suitable

form

liquid

form

liquid

form

liquid

form

liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

-10 to -25°C

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100


Klasa składowania

12 - Non Combustible Liquids

Co się dzieje?

nwg

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable



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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–5 of 5 Questions  
  1. How long can this be stored in the refrigerator for?

    1 answer
    1. This product is not assigned an expiration date. Products that have no expiration date are robust in nature and extremely stable in the original sealed container. These products will have no dating reported on either the label or the Certificate of Analysis but are covered by a warranty period of 1 year from the date of product shipment. The complete Product Dating statement may be reviewed at the link below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/190/408/product-dating-information-ms.pdf .

      Helpful?

  2. What are the concentrations of AAs?

    1 answer
    1. The concentration of amino acids can be found in the formulation documentation, please view the formulation document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/210/498/m5550for.pdf.

      Helpful?

  3. Can this be used for cell free expression on E.coli system?

    1 answer
    1. This product is intended for use in mammalian cell culture, not for bacteria.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. Can Product M5550, MEM Amino Acids (50X) solution contain precipitates?

    1 answer
    1. Product M5550, MEM Amino Acids (50X) has a specification as a clear solution.  This product can have a slight precipitate that forms at 2-8°C.  This precipitate will disappear when warmed to room temperature or when diluted to 1X.  There is no affect on the product quality.

      Helpful?

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