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M6444

Sigma-Aldrich

Anti-Mitofusin 2 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-MARF, Anti-Mfn2, Anti-KIAA0214, Anti-CMT2A2, Anti-CMT2A, Anti-CPRP1
Numer MDL:
NACRES:
NA.41

pochodzenie biologiczne

rabbit

Poziom jakości

200

białko sprzężone

unconjugated

forma przeciwciała

affinity isolated antibody

antibody product type

primary antibodies

klon

polyclonal

forma

buffered aqueous solution

masa cząsteczkowa

antigen ~86 kDa

species reactivity

rat, mouse, human

stężenie

~1 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using HeLa human epitheloid carcinoma cell lysate
western blot (chemiluminescent): 0.5-1 μg/mL using extract of mouse and rat brain mitochondria

numer dostępu UniProt

transport

dry ice

temp. przechowywania

−20°C

Gene Information

human ... MFN2(9927)
mouse ... Mfn2(170731)
rat ... Mfn2(64476)

Opis ogólny

Anti-Mitofusin 2 is produced in rabbit using as immunogen a synthetic peptide corresponding to human mitofusin 2 with an N-terminal added cysteine, conjugated to KLH. Mitofusins (Mfn1 and Mfn2) form homotypic and heterotypic complexes for fusion in mitochondria.
Mitofusin-2 (MFN2) is an outer mitochondrial membrane protein.

Immunogen

synthetic peptide corresponding to amino acid residues 557-576 of human mitofusin 2 with an N-terminal added cysteine, conjugated to KLH. The corresponding sequence differs by one and two amino acids in rat and mouse mitofusin 2, respectively.

Zastosowanie

Anti-Mitofusin 2 antibody produced in rabbit has been used for Western blotting.
Anti-Mitofusin 2 antibody has been used in immunoblotting. It may also be used in immunoprecipitation.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Działania biochem./fizjol.

MFN2 is essential for the maintenance of mitochondrial network and controls mitochondrial metabolism. Mfn2-dependent regulatory mechanism is disturbed in obesity by reduced Mfn2 expression. Mutations in MFN2 cause Charcot Marie-Tooth neuropathy type 2A, a neurological disorder that results from degeneration of axons in peripheral nerves.
Mitofusin-2 (MFN2) plays an important role as a mitochondrial fusion protein and participates in many cell processes. It is mainly involved in regulating metabolic processes in the mitochondria. MFN2 prevents the formation of reactive oxygen species and controls endoplasmic reticulum stress. Mutations in the gene encoding this protein have been linked to diabetes and obesity.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Kod klasy składowania

10 - Combustible liquids

WGK

WGK 3

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Certificate of Analysis

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Certificate of Origin

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SDS

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