N5386

Nuclease micrococcal from Staphylococcus aureus

100-300 units/mg protein

• Synonim(y): Endonuclease micrococcal, MNase, Micrococcal endonuclease
• Numer CAS: 9013-53-0
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-748-0
• MDL number: MFCD00131731
• Numer WE: 3.1.31.1 (BRENDA, IUBMB)
• Specific activity: 100-300 units/mg protein
• Biological source: Staphylococcus aureus

Właściwości

• biological source: Staphylococcus aureus
• Quality Segment: 200
• form: powder
• specific activity: 100-300 units/mg protein
• composition: Protein, ≥40% E1%/280 (, Balance primarily sodium acetate)
• technique(s): DNA extraction: suitable, DNA purification: suitable
• input: bacteria and yeast sample(s)
• suitability: suitable for molecular biology
• application(s): cell analysis
• storage temp.: −20°C
• Gene Information: Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)

Opis

Application

Micrococcal Nuclease can be used for the digestion of chromatin to identify the location of nucleosomes.

Nuclease micrococcal from Staphylococcus aureus has been used in a study to monitor hybridization reactions with DNA. It has also been used in a study to investigate the optimal conditions for assay of staphylococcal nuclease.

Biochem/physiol Actions

Hydrolyzes 5′-phosphodiester bonds of DNA.

Other Notes

Note: One μmolar unit = approx. 85 A₂₆₀ units, where an A₂₆₀ unit is equivalent to a ΔA₂₆₀ of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• Co się dzieje?: WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• PPE (środki ochrony indywidualnej): Eyeshields, Gloves, type N95 (US)