This product can be used to remove endotoxins from solutions. A protocol for use and information on regeneration of the resin are provided in the Product Information Sheet.
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P1411
Polymyxin B-Agarose
aqueous glycerol suspension
Synonim(y):
PMB
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| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 5 mL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 782,00 zł | |
| 10 mL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1510,00 zł |
Informacje o tej pozycji
782,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócbiological source
synthetic (organic)
Quality Level
form
aqueous glycerol suspension
extent of labeling
~1 mg per mL
technique(s)
affinity chromatography: suitable
matrix
Cross-linked 4% beaded agarose
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
capacity
200-500 μg/mL binding capacity (lipopolysaccharide)(from Escherichia coli serotype 0128:B12)
suitability
suitable for chromatography
storage temp.
2-8°C
Application
Polymyxin B-agarose is used in protein chromatography, affinity chromatography and specialty resins. Polymyxin B-agarose has been used to study periodontitis and endodontic infections as well as obesity and type 2 diabetes.
Useful for removal of endotoxins from solutions
Physical form
Suspension in 50% glycerol containing 0.02% sodium azide
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Ta pozycja | |||
|---|---|---|---|
| technique(s) affinity chromatography: suitable | technique(s) - | technique(s) - | technique(s) HPLC: suitable, gas chromatography (GC): suitable |
| biological source synthetic (organic) | biological source - | biological source - | biological source - |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
| form aqueous glycerol suspension | form ready-to-use solution | form lyophilized powder | form - |
| suitability suitable for chromatography | suitability suitable for (Fluorescent detection of gram-negative bacteria), suitable for (Microbiome), suitable for microbiology | suitability - | suitability - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. - |
Klasa składowania
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Product Information Sheet
Martín López et al.
Journal of immunology (Baltimore, Md. : 1950), 170(5), 2409-2416 (2003-02-21)
Macrophages infected with Mycobacterium tuberculosis undergo increased rates of apoptosis. Important objectives are to define the microbial factors that cause apoptosis, the mechanisms involved and the impact on infection. The 19-kDa M. tuberculosis glycolipoprotein (p19) is both cell wall-associated and
A C Issekutz
Journal of immunological methods, 61(3), 275-281 (1983-07-29)
Endotoxins liberated by gram-negative bacteria are frequent contaminants of aqueous and physiological solutions. Because of their potent biological effects in vivo and in vitro, it is often necessary to eliminate even minute quantities of endotoxin from such solutions. A process
C Moreno et al.
Clinical and experimental immunology, 76(2), 240-245 (1989-05-01)
We show here that purified lipoarabinomannan (LAM) from Mycobacterium tuberculosis can cause the release of tumour necrosis factor (TNF) in vitro from human blood monocytes and activated mouse peritoneal macrophages, and the production of TNF in vivo in mice pretreated
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| P1411-10ML | 04061834354925 |
| P1411-5ML | 04061834354932 |
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What is the application of this polymyxin-B agarose? can we re-use it for several times? And if yes, how can we regenerate it? thanks!
1 answer-
Helpful?
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What polypropylene column should the we use according to the protocol to wash the resin prior to use?
1 answer-
For washing the resin prior to use, the customer can use the following options:
1. C2603 Mini-Columns: These have a capacity of 0.5 mL and an overall height of 1.9 inches.
2. C2728 Mini-Columns: These have a capacity of 2.4 mL and an overall height of 1.5 inches.
The washing procedure using Polymyxin B Agarose (Product No. P1411) can be carried out in a column or in a batch method. For a column, the customer can use disposable mini columns, such as Product Nos. C2603 (0.5 ml) and C2728 (5.0 ml). If a larger column is needed, the customer can use a polypropylene syringe barrel or use the batch method.
In the batch method, the solution is incubated with the polymyxin B agarose for a minimum of 30 minutes with rotation in either a 15 ml or 50 ml polypropylene tube. The tube is then centrifuged, and the supernatant contains the protein of interest with decreased endotoxin that has been bound to the beads and pelleted in the tube.
Helpful?
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What methods can be used to remove endotoxin from a product?
1 answer-
The product is not tested for endotoxin; however, P1411 may be used to strip endotoxin. The P1411 datasheet includes a suggested protocol for this process. The protocol involves washing the resin, equilibrating the column, dissolving the sample in buffer, eluting with endotoxin-free buffer, and collecting the fractions. The collected fractions containing the protein should then be lyophilized. The polymyxin B agarose can be regenerated at least 10 times, and the resin can be stored in 25% ethanol at 4°C
Helpful?
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