P1484
Anti-p21WAF1/Cip1antibody, Mouse monoclonal
clone CP74, purified from hybridoma cell culture
Synonim(y):
Anti-CDKN1A, Anti-Cip1, Anti-WAF1, Anti-p21
Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.
Wybierz wielkość
0.2 ML
2350,00 zł
2350,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Rekombinowane, niezawierające konserwantów przeciwciało jest dostępne dla Twojego celu. Wypróbuj ZRB1141
Informacje o tej pozycji
Conjugate:
unconjugated
Clone:
CP74, monoclonal
Application:
ARR, ELISA (i), IP, WB
Citations:
41
Przejdź do
Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomócbiological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
CP74, monoclonal
form
buffered aqueous solution
mol wt
antigen 21 kDa
species reactivity
mouse, human
concentration
~1 mg/mL
technique(s)
immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 5-10 μg/mL using LNCap cells (human prostate adenocarcinoma) treated with MG132 proteosome inhibition.
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CDKN1A(1026)
mouse ... Cdkn1a(12575)
1 of 4
Ta pozycja | |||
|---|---|---|---|
| Gene Information human ... CDKN1A(1026) | Gene Information human ... PPP1R13L(10848) | Gene Information human ... CDK4(1019) | Gene Information human ... CDKN1B(1027) |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| species reactivity mouse, human | species reactivity mouse, human | species reactivity rat, mouse, human | species reactivity rat, mouse, human |
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form ascites fluid | antibody form affinity isolated antibody |
| clone CP74, monoclonal | clone LXO49.3, monoclonal | clone DCS-31, monoclonal | clone polyclonal |
| biological source mouse | biological source mouse | biological source mouse | biological source rabbit |
General description
Monoclonal Anti-p21WAF1/Cip1 (mouse IgG2b isotype) is derived from the CP74 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with recombinant human p21WAF1/Cip1. p21WAF1/Cip1 is 21 kDa and designated as Cip1, WAF1, Sdi1, Pic1, CAP20.
Mouse monoclonal clone CP74 anti-p21WAF1/Cip1 antibody reacts specifically with p21WAF1/Cip1. Two families of CKIs have been identified. The p21WAF1/Cip1 family contains p21WAF1/Cip1, p27Kip1 and p57Kip2.
Immunogen
recombinant human p21WAF1/Cip1.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Western Blotting (1 paper)
Immunofluorescence (1 paper)
Western Blotting (1 paper)
Mouse monoclonal clone CP74 anti-p21WAF1/Cip1 antibody may be used for ELISA, immunoblotting (21 kDa) and immunoprecipitation (p21/cyclin A/Cdk2 complexes, native and denatured). The availability of a monoclonal antibody reacting specifically with p21WAF1/Cip1 enables the subcellular detection and localization of p21WAF1/Cip1 and the measurement of relative differences in p21WAF1/Cip1 levels as a function of cell cycle phase.
Biochem/physiol Actions
Regulation of cell cycle progression in eukaryotic cells depends on the expression of proteins called cyclins. These proteins form complexes with several different cyclin dependent kinases (CDKs). The catalytic activity of cyclin dependent kinases (CDK) is regulated by two general mechanisms: protein phosphorylation and association with regulatory subunits, which include the cyclins and the CDK inhibitors (CKIs). These inhibit all kinases involved in the G1/S transition. The the p16INK4a family, which includes p15INK4b, p16INK4a, p18INK4c and p19INK4d, inhibits Cdk4 and Cdk6 specifically.
The p21WAF1/Cip1 inhibit all kinases involved in the G1/S transition of cell cycle. p21WAF1/Cip1 binds tightly to the G1 and S phase kinases, cyclin E/Cdk2, cyclin D/Cdk4, and cyclin A/Cdk2, and effectively inhibits their activity, whereas p21WAF1/Cip1 is relatively a poor inhibitor of the G2/M phase kinase cyclin B/Cdc2. In addition, p21WAF1/Cip1 inhibits proliferating cell nuclear antigen (PCNA), binds to and inhibits inactivation of Rb which is essential for cell cycle progression, and may also interact with E2F and protect cells against p53-mediated apoptosis.
Physical form
Solution in phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Ta strona może zawierać tekst przetłumaczony maszynowo.
Nie możesz znaleźć właściwego produktu?
Wypróbuj nasz Narzędzie selektora produktów.
related product
Klasa składowania
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Lu Deng et al.
Cell death & disease, 9(12), 1143-1143 (2018-11-18)
Myristoylation is one of key post-translational modifications that involved in signal transduction, cellular transformation and tumorigenesis. Increasing evidence demonstrates that targeting myristoylation might provide a new strategy for eliminating cancers. However, the underlying mechanisms are still yielded unclear. In this
Tumor susceptibility of p21Waf1/Cip1-deficient mice
Martin- Caballero J, et al.
Cancer research, 61(16), 6234-6238 (2001)
Isabel de Pedro et al.
Cell death & disease, 9(11), 1094-1094 (2018-10-27)
The epidermis is a self-renewal epithelium continuously exposed to the genotoxic effects of ultraviolet (UV) light, the main cause of skin cancer. Therefore, it needs robust self-protective mechanisms facing genomic damage. p53 has been shown to mediate apoptosis in sunburn
T Feng et al.
Oncogenesis, 6(1), e294-e294 (2017-01-31)
TGF-β signaling in liver cells has variant roles in the dynamics of liver diseases, including hepatocellular carcinoma (HCC). We previously found a correlation of high levels of the important endogenous negative TGF-β signaling regulator SMAD7 with better clinical outcome in
Yang Yang et al.
Cell death & disease, 9(9), 886-886 (2018-09-01)
The tumor suppressor p53 plays a pivotal role in the protection against cancer. Increasing evidence suggests that long noncoding RNA (lncRNA) plays an important role in the regulation of the p53 pathway, however, the detailed mechanisms remain to be further
Powiązane treści
Active Filters
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej