immunocytochemistry: suitable using 4% paraformaldehyde-0.5% Triton X-100 fixation, indirect ELISA: suitable, microarray: suitable, western blot: 5-10 μg/mL using whole extract of rat brain
The PTEN (phosphatase and tensin homolog) gene is mapped to human chromosome 10q23. It shares sequence homology with protein tyrosine phosphatases and the cytoskeletal protein tensin.
Immunogen
synthetic peptide corresponding to the C-terminus of rat PTEN (amino acids 386-403).
Biochem/physiol Actions
The PTEN (phosphatase and tensin homolog) gene encodes a encodes a dual-specificity phosphatase that functions as a tumor suppressor by inhibiting phosphoinositide-3-kinase–protein kinase B/Akt (PI3K/PKB/Akt) signaling pathway, a crucial regulator of cell growth and survival and by negatively regulating the interactions of cell with the extracellular matrix. Mutations in this gene have been associated with several human cancers. Germline mutations in this gene have also been linked to Cowden disease (CD), Lhermitte-Duclos disease (LDD) and Bannayan-Zonana syndrome (BZS), which are characterized by increased susceptibility to tumor formation and developmental defects.
The epitope recognized by the antibody resides within amino acids 386-403 of rat PTEN.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.
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