Endotoxin-free products are all typically filtered with a 0.1 micron pore size filter. Sterile filtered products typically have a 0.2 or 0.22 pore size filter.
R8758
RPMI-1640 Medium
With ʟ-glutamine, sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
RPMI-1640 Medium
Synonim(y):
Roswell Park Memorial Institute 1640 medium
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Wybierz wielkość
100 ML
84,60 zł
500 ML
179,00 zł
1 L
311,00 zł
6 X 500 ML
913,00 zł
6 X 1 L
1760,00 zł
24 X 500 ML
3190,00 zł
84,60 zł
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Pozwól nam pomócNazwa produktu
RPMI-1640 Medium, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Poziom jakości
sterylność
sterile-filtered
Formularz
liquid
metody
cell culture | mammalian: suitable
zanieczyszczenia
endotoxin, tested
komponenty
NaHCO3: yes
phenol red: yes
sodium pyruvate: no
HEPES: no
L-glutamine: yes
Warunki transportu
ambient
temp. przechowywania
2-8°C
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Ta pozycja | R0883 | R7638 | R7509 |
|---|---|---|---|
| form liquid | form liquid | form liquid | form liquid |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| components NaHCO3: yes | components sodium pyruvate: no | components sodium pyruvate: no | components phenol red: no |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
Opis ogólny
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
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Kod klasy składowania
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Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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When the medium waas sterile-filtered, which pore size was used?
1 answer-
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Good Morning, does RPMI medium R8758 which contains sodium bicarbonate, affect cell suspension culture of megakaryoblastic cell line DAMI? I mean, sodium bicarbonate can significantly modify cell behavior or his effects can be tolerated?
1 answer-
This product has not been validated for use with the megakaryoblastic cell line DAMI. However, literature indicates compatibility with this cell line. The following citation may be of interest: PLoS One. 2020 Apr 7;15(4):e0230507.
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I bought this product and realized that it does not contain Hepes. Can I add hepes?
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This product utilizes a bicarbonate buffering system. However, there are no anticipated problems with the addition of HEPES. We do offer RPMI formulations containing both sodium bicarbonate and HEPES, such as Products R5886 & R7638.
Please see the RPMI formulation tables at the link below: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/203/777/r8758for.pdf
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Considering that I have to use an RPMI medium containing 4mM of l-Glutamine and 10mM of non-essential amino acids, can I use the product R8748 without adding anything?
1 answer-
The information you are requesting can be supplied by our Technical Service team who can assist you further. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, and click on "Product Technical Inquires" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.
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buenos dias, llevo años usando vuestro producto RPMI 1640, y me gustaría saber la composición del medio para ver si hay algun metal/producto que no puede utilizar con un suplemento que quiero introducir. Muchas gracias
1 answer-
Please see the link below to review the RPMI formulation tables. This product is listed in the second column of page 2:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/203/777/r8758for.pdfHelpful?
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Can Product R8758, RPMI-1640 Medium, be frozen?
1 answer-
We do not recommend freezing cell culture media. A precipitate may form upon thawing. Also, this can cause a loss of amino acids, which may yield low cell growth.
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Does Product R8758, RPMI-1640 Medium, contain HEPES?
1 answer-
There is no HEPES in this formulation of RPMI. We do offer RPMI containing HEPES as Product No's. R5886 and R4130. The formulation of RPMI and other classical media can be found on the Cell Culture Web Page.
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Does Product R8758, RPMI-1640 Medium, contain phenol red?
1 answer-
This media contains phenol red. The formulation of RPMI and other classical media can be found on the Cell Culture Web Page.
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Can I add the glutamine dipeptide to Product R8758, RPMI-1640 Medium?
1 answer-
This formulation of RPMI already contains L-glutamine at 0.3 g/L. It does not require further supplementation with L-glutamine or the L-glutamine dipeptide (Product No. G8541).
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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