The buffers used in the experiment were 0.1M citrate/0.2M phosphate with a pH of 5.6 and 0.3 M Glycine with a pH of 10.6. The stop solution was used with a standard curve ranging from 1-1000 nM.
Przejdź do
SAE0052
Chitotriosidase human
recombinant, expressed in HEK 293 cells
Synonim(y):
Chitinase-1, Chitotriosidase-1
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
10 μg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1330,00 zł |
1330,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócrecombinant
expressed in HEK 293 cells
Quality Segment
assay
98% (SDS-PAGE)
form
lyophilized powder
specific activity
≥15,000 units/μg protein
mol wt
50 kDa
shipped in
ambient
storage temp.
−20°C
General description
Chitotriosidase (CT) is a human chitinase with catalytic activity upon the substrate chitin, a polymer of ?-1,4-N-acetylglucosamine. The mature CT consists of 445 amino acids, and contains a potential N-glycosylation site as well as three intrachain disulfide bonds.
Application
The enzymatic activity of recombinant human CT is measured by its ability to hydrolyze the substrate 4-Methylumbelliferyl beta -D-N,N′,N”-triacetylchitotriose (Cat. # M5639). This enzymatic hydrolysis releases 4-methylumbelliferone (4MU), which upon ionization in basic pH, can be measured fluorimetrically at an excitation wavelength of 340 nm and an emission wavelength of 465 nm. The use of fluorimetric substrates provides a very sensitive detection system.
Biochem/physiol Actions
CT is currently the most reliable biomarker in Gaucher disease - serum and plasma CT activity is usually measured as the first step in diagnosis of Gaucher disease. Monitoring chitotriosidase activity is widely used during treatment of this pathology by enzyme replacement therapy. Its elevated plasma level reflects gradual intralysosomal accumulation in Gaucher cells (lipid-loaded macrophages). Macrophages overloaded by the enzyme accumulated in lysosomal material (lipids) were shown to secrete chitotriosidase; its increased expression was noted in several lysosomal storage diseases and atherosclerosis.
Physical form
Supplied as a lyophilized powder containing phosphate buffered saline
Other Notes
AKLVCYFTNWAQYRQGEARFLPKDLDPSLCTHLIYAFAGMTNHQLSTTEWNDETLYQEFNGLKKMNPKLKTLLAIGGWNFGTQKFTDMVATANNRQTFVNSAIRFLRKYSFDGLDLDWEYPGSQGSPAVDKERFTTLVQDLANAFQQEAQTSGKERLLLSAAVPAGQTYVDAGYEVDKIAQNLDFVNLMAYDFHGSWEKVTGHNSPLYKRQEESGAAASLNVDAAVQQWLQKGTPASKLILGMPTYGRSFTLASSSDTRVGAPATGSGTPGPFTKEGGMLAYYEVCSWKGATKQRIQDQKVPYIFRDNQWVGFDDVESFKTKVSYLKQKGLGGAMVWALDLDDFAGFSCNQGRYPLIQTLRQELSLPYLPSGTPELEVPKPGQPSEPEHGPSPGQDTFCQGKADGLYPNPRERSSFYSCAAGRLFQQSCPTGLVFSNSCKCCTWNGHHHHHHHHGQ
One unit is defined as the amount of CT required to produce 1 pmol of 4-Methylumbelliferone from 4-Methylumbelliferyl beta -D-
N′,N”-triacetylchitotrioside per minute at 25 °C, pH 5.6.
N′,N”-triacetylchitotrioside per minute at 25 °C, pH 5.6.
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Ta pozycja | |||
|---|---|---|---|
| description recombinant, expressed in HEK 293 cells | description for serum, plasma and cell culture supernatants | description Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution | description ≥98% (HPLC) |
| assay 98% (SDS-PAGE) | assay - | assay - | assay ≥98% (HPLC) |
| specific activity ≥15,000 units/μg protein | specific activity - | specific activity - | specific activity - |
| recombinant expressed in HEK 293 cells | recombinant - | recombinant - | recombinant - |
| form lyophilized powder | form - | form buffered aqueous glycerol solution | form powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| shipped in ambient | shipped in wet ice | shipped in wet ice | shipped in - |
Klasa składowania
10 - Combustible liquids
Co się dzieje?
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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