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T0678
Anti-Tryptophan Hydroxylase Antibody
mouse monoclonal, WH-3
Synonim(y):
Anti-TPH
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
100 μL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 2400,00 zł |
0.2 mL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 2960,00 zł |
Informacje o tej pozycji
Conjugate:
unconjugated
Clone:
WH-3, monoclonal
Application:
ARR, ELISA (i), IHC, WB
Citations:
74
2400,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
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Pozwól nam pomócNazwa produktu
Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse, clone WH-3, ascites fluid
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
WH-3, monoclonal
mol wt
antigen 55 kDa
contains
15 mM sodium azide
species reactivity
human, monkey, rabbit, rat
technique(s)
immunohistochemistry: suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1:1,000 using rabbit pineal gland extract
isotype
IgG3
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... TPH1(7166), TPH2(121278)
rat ... Tph1(24848), Tph2(317675)
General description
Monoclonal Anti-Tryptophan Hydroxylase (mouse IgG3 isotype) is derived from the WH-3 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Tryptophan hydroxylase (TPH; L-tryptophan tetrahydropteridine:oxygen oxidoreductase; EC 1.14.16.4), the rate-limiting enzyme for the synthesis of serotonin, is a very specific enzyme found only inside serotoninergic neurons.
The tryptophan hydroxylase 1 gene (TPH1) is a 444 amino acid protein encoded by the gene spanning 29 kbp with11 exons on human chromosome 11p15.3-p14. The enzyme is expressed in the gut, spleen, thymus, pituitary and also in the pineal gland.
Immunogen
recombinant rabbit tryptophan hydroxylase.
Application
Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunohistochemistry
- immunoblot
- immunocytochemistry
Biochem/physiol Actions
The tryptophan hydroxylase 1 gene (TPH1) catalyzes the synthesis of 5-hydroxytryptophan, which is a precursor to the neurotransmitter serotonin. Variation in the gene expression affects prefrontal cortex activation during response inhibition. Mutation in the TPH1 gene leads to schizophrenia, irritable bowel syndrome (IBS) in women, suicidal behavior and depressive disorders. The encoded protein plays a vital role in regulation of cardiovascular function.
This antibody reacts specifically with tryptophan hydroxylase.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Ta pozycja | |||
|---|---|---|---|
| description clone WH-3, ascites fluid | description affinity isolated antibody, buffered aqueous glycerol solution | description clone WH-3, purified from hybridoma cell culture | description affinity isolated antibody |
| Gene Information human ... TPH1(7166), TPH2(121278) | Gene Information human ... TPH1(7166) | Gene Information - | Gene Information human ... TPH1(7166) |
| conjugate unconjugated | conjugate unconjugated | conjugate - | conjugate unconjugated |
| biological source mouse | biological source sheep | biological source mouse | biological source rabbit |
| species reactivity human, monkey, rabbit, rat | species reactivity mammals, human | species reactivity mouse, human, rabbit, sheep, rat | species reactivity rat, mouse, human |
| antibody form ascites fluid | antibody form affinity isolated antibody | antibody form purified from hybridoma cell culture | antibody form affinity isolated antibody |
| clone WH-3, monoclonal | clone polyclonal | clone WH-3, monoclonal | clone polyclonal |
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Klasa składowania
10 - Combustible liquids
WGK
nwg
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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What is the Department of Transportation shipping information for this product?
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Do you have an immunohistochemistry protocol for use with Product T0678, Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse?
1 answer-
Free floating sections (4% paraformaldehyde fixation)1. Incubate 30 minutes at room temperature in 0.3% Triton X-100, 5% normal serum.2. Incubate with the antibody diluted 1:10,000 in PBS, 0.3% Triton X-100, 3.5% normal serum and 0.05% BSA for 36-48 hours.3. Rinse 3 times for 5 minutes each in PBS.4. Add the biotinylated anti-mouse IgG3 diluted in PBS containing 0.3% Triton X-100 and 3.5% normal serum for 1 hour at room temperature.5. Rinse 3 times for 5 minutes each in PBS.6. Process with Avidin Biotin-HRP.7. Complete the reaction with a 2-10 minute incubation in PBS containing 0.05% DAB and 0.015% hydrogen peroxide (H2O2).8. Mount the sections on gel coated slides and air dry.
Helpful?
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Does Product T0678, Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse, cross-react with tyrosine or phenylalanine hydroxylases?
1 answer-
This antibody, unlike some other antibodies to tryptophan hydroxylases (WH) in the market, does not cross react with tyrosine hydroxylase (TH) or with phenylalanine hydroxylase (PH). The epitope recognized by the antibody is not located in the Pterin Binding Region, which is homologous among PH, TH and WH. The specificity to WH is confirmed by the single band obtained at the 55KD region, while TH has a higher MW (60-68KD), depending on the submit) and PH has a lower MW. It has also been confirmed by collaborating experts. See Journal of Neuroscience Methods, 114(2), 205-212 (2002).
Helpful?
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Can Product T0678, Monoclonal Anti-Tryptophan Hydroxylase antibody produced in mouse, be used to stain mouse brain sections?
1 answer-
This antibody has been used successfully on mouse brain slices in an immunohistochemical application. The IHC results from an experiment employing T0678 at 1:500 in mouse brain slice staining clearly showed the dorsal nuclear raphe (which are the serotonergic neurons).
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