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T3038

Trizma® hydrochloride solution

1 M, BioReagent, Molecular Biology

Synonim(y):

Tris hydrochloride solution

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Rozmiar/SKUDostępnośćCena netto
1 L
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381,00 zł

Informacje o tej pozycji

NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12161700
MDL number:

381,00 zł


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grade

Molecular Biology

Quality Segment

sterility

0.2 μm filtered

product line

BioReagent

form

solution

concentration

1 M

impurities

DNase, RNase, NICKase and protease, none detected

pH

8.0

application(s)

agriculture

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

General description

Trizma® hydrochloride solution is a useful biological buffer.

Application

Trizma® hydrochloride solution may be used in the following studies:
  • As buffer for the 2-D electrophoresis of rat fibroblast cell.
  • As buffer for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length).
  • Selective immunoprecipitation of biotin-labeled DNA with antibiotin IgG and Staphylococcus sp.
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Other Notes

Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
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Ta pozycja
SBR00051T2569T2444
description

1 M, BioReagent, Molecular Biology

description

pH 8.5, DNase and Microbial DNA free, 10 mM

description

pH 7.8, BioPerformance Certified, 1 M, suitable for cell culture

description

pH 7.6, BioPerformance Certified, 1 M, suitable for cell culture

grade

Molecular Biology

grade

-

grade

Molecular Biology, BioPerformance Certified

grade

Molecular Biology, BioPerformance Certified

concentration

1 M

concentration

10 mM

concentration

1 M

concentration

1 M

product line

BioReagent

product line

-

product line

-

product line

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

solution

form

-

form

solution

form

solution

application(s)

agriculture

application(s)

-

application(s)

-

application(s)

-


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Klasa składowania

10 - Combustible liquids

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

PPE (środki ochrony indywidualnej)

Eyeshields, Gloves, type ABEK (EN14387) respirator filter



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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the tris molarity in Trizma hydrochloride buffer cat# 41573 ?

    1 answer
    1. Trizma is a registered trademark. The concentration of this product is between 0.95 - 1.05 M. The exact concentration is listed on the lot specific Certificate of Analysis: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/T3038-1LSLCQ0820.pdf.

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