T9026

Sigma-Aldrich

Monoclonal Anti-α-Tubulin antibody produced in mouse

enhanced validation

clone DM1A, ascites fluid

Synonim(y):
Monoclonal Anti-α-Tubulin
NACRES:
NA.41

Poziom jakości

200

pochodzenie biologiczne

mouse

forma przeciwciała

ascites fluid

antibody product type

primary antibodies

klon

DM1A, monoclonal

masa cząsteczkowa

antigen ~50 kDa

zawiera

15 mM sodium azide

species reactivity

bovine, rat, yeast, human, mouse, chicken, fungi, amphibian

rozszerzona walidacja

independent ( antibodies)
Learn more about Antibody Enhanced Validation

zastosowania

indirect immunofluorescence: 1:500 using cultured chicken fibroblasts
western blot: 1:500 using human or chicken fibroblasts

izotyp

IgG1

białko sprzężone

unconjugated

Wyróżniony przemysł

Research Pathology

numer dostępu UniProt

wysyłka w ciągu

dry ice

temp. przechowywania

−20°C

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

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Opis ogólny

α-tubulin, also called tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α-tubulin family, and contains 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000.
Monoclonal Anti-α-Tubulin (mouse IgG1 isotype) is derived from the DM1A hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Purified chick brain microtubules were used as immunogen. The isotype is determined by a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents, Product Number ISO2. The product is Protein A purified Monoclonal Anti-α-Tubulin antibody conjugated to fluorescein isothiocyanate, isomer I. It is purified by gel filtration and contains no detectable free FITC. Anti-α-Tubulin FITC antibody, Mouse monoclonal specifically recognizes an epitope in the carboxy terminal part of α-tubulin. It localizes α-tubulin in human, monkey, bovine, chicken, goat, murine, rat, gerbil, hamster, rat kangaroo, amphibia, sea urchin, trypanosome, yeast, fungi and tobacco.

Specyficzność

The antibody is specific for α-tubulin in immunoblotting assays and may be used for localization of α-tubulin in cultured cells or tissue sections. The antibody reacts best with chicken fibroblasts.

Immunogen

microtubules from chicken embryo brain.

Zastosowanie

Monoclonal Anti-α-Tubulin antibody produced in mouse has been used in western blot analysis and protein expression analysis.
Monoclonal Anti-α-Tubulin antibody produced in mouse has been used in the detection of α-Tubulin:
  • in human osteosarcoma and in breast cancer cell lines by western blotting
  • in HeLa cells by immunofluorescence microscopy
  • by immunostaining
  • immunohistochemical detection in Xenopus embryos

Działania biochem./fizjol.

Tubulin α 4a (TUBA4A) mutation is associated with amyotrophic lateral sclerosis (ALS).
α/β-Tubulin is the major building block of microtubules. These intracellular, hollow, cylindrical, filamentous structures are present in virtually all eukaryotic cells. Self-assembly of α/β-tubulin leads to polar microtubular structures built from linearly arranged strings of alternatively α- and β-tubulin pairs pointing in the same direction. Microtubules function as structural and mobile elements in mitosis, intracellular transport, ciliary flagellar motility and generation and maintenance of cell shape. α/β-Tubulin and γ-tubulin are members of the tubulin superfamily of proteins. α/β-Tubulin is a heterodimer which consists of one α-tubulin chain and one β-tubulin chain; each subunit has a molecular weight of 55 kDa and they share considerable homolog.

Przechowywanie i stabilność

For continuous use, store at 2-8 °C for up to one month.
For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

10 - Combustible liquids

WGK Germany

WGK 3

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Certyfikat analizy

Świadectwo pochodzenia

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the immunogen for Monoclonal Anti-α-Tubulin antibody, clone DM1A?

    The immunogen for Monoclonal Anti-α-Tubulin antibody produced in mouse, clone DM1A is microtubules from chicken embryo brain.  This antibody recognizes an epitope located at the C-terminal end of the α-tubulin isoform (amino acids 426-430) as noted in the following references:Breitling, F., and Little, M., J. Molec. Biol., 189, 367-370 (1986).Wolff, A., et al., Biol. Cell, 63, 319-326 (1988).Serrano, L., et al., Anal. Biochem., 159, 253-259 (1986).

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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    Ask a Scientist here.

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Rahman M, et al.
Breast Cancer Research and Treatment, 113(2), 217-230 (2009)
p38 Mitogen-activated protein kinase-and HuR-dependent stabilization of p21Cip1 mRNA mediates the G1/S checkpoint.
Lafarga V, et al.
Molecular and Cellular Biology, 29(16), 4341-4351 (2009)
Unconventional functions of microtubule motors.
Muresan V and Muresan Z
Archives of Biochemistry and Biophysics, 520(1), 17-29 (2012)
The self-assembly and dynamic structure of cytoskeletal filaments.
Alberts B, et al.
Molecular biology of the Cell. 4th Edition. (2002)
E2F1 regulates autophagy and the transcription of autophagy genes.
Polager S, et al.
Oncogene, 27(35), 4860-4860 (2008)
Produkty
Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.
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High titer lentiviral particles including beta-actin, alpha-tubulin and vimentin used for live cell analysis of cytoskeleton structure proteins.
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