T9701

TRAIL human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE and HPLC), lyophilized powder

• Synonim(y): TL2, Apo2 Ligand, Apo2L, TNF-related apoptosis-inducing ligand
• Numer CAS: 2244730-93-4
• Numer MDL: MFCD01867158
• NACRES: NA.32

Właściwości

• Poziom jakości: 200
• pochodzenie biologiczne: human
• rekombinowane: expressed in E. coli
• opis: extracellular domain
• próba: ≥98% (SDS-PAGE and HPLC)
• forma: lyophilized powder
• masa cząsteczkowa: 19.6 kDa; predicted mol wt ~21 kDa
• technique(s): immunofluorescence: suitable
• zanieczyszczenia: endotoxin, tested
• numer dostępu UniProt: P50591
• temp. przechowywania: −20°C
• Gene Information: human ... TNFSF10(8743)

Opis

Zastosowanie

Human TRAIL can be used as a selection agent for treating human cells for phenotypic enrichments. The product can also be used in chemical transfection for identifying Small RNA Modulators of TRAIL-Induced Apoptosis Pathway by utilizing libraries of synthetic small interfering RNA (siRNA) and microRNAs (miRNA).

TRAIL human has been used in immunofluorescence analyses, to study the delocalization of melanoma antigen-D2 in the nucleoplasm after a cellular stress.

Działania biochem./fizjol.

Type II transmembrane protein of the TNF superfamily. Ligand for apoptosis-inducing death receptors DR4 and DR5, decoy receptors DcR1 and DcR2, and osteoprotegerin. Approximately 28% amino acid sequence identity to FAS ligand. Human and mouse TRAIL share 65% sequence identity.

TRAIL is a cytokine with anti-inflammatory, and antitumor activity. It is expressed in various cells including immune cells, vascular cells and adipocytes. TRAIL is associated with vascular endothelial cell protection.

TNF-related apoptosis-inducing ligand (TRAIL) is a type II transmembrane protein that belongs to TNF family. The product present in both soluble as well as membrane-bound form, can induce rapid apoptosis of various transformed cell lines but not the normal cells. Additionally, the product can also induce apoptotic cell death in TRAIL-sensitive U343MG cells.

Postać fizyczna

Lyophilized from phosphate buffered saline

Komentarz do analizy

The biological activity is determined by its ability to induce apoptotic cell death in TRAIL-sensitive LN-18 cells.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 13 - Non Combustible Solids
• WGK: WGK 3
• Temperatura zapłonu °F: Not applicable
• Temperatura zapłonu °C: Not applicable