ZRB1012

Anti-Choline Acetyltransferase (ChAT) Antibody, clone 2K16, ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonim(y): CHOACTase, ChAT, Choline acetylase, EC: 2.3.1.6
• NACRES: NA.43
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: 2K16, recombinant monoclonal, monoclonal
• Application: IF, IHC, WB
• Citations: 2

Właściwości

• biological source: rabbit (recombinant)
• Quality Segment: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2K16, recombinant monoclonal, monoclonal
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 82.54 kDa, observed mol wt ~65 kDa
• species reactivity: rat, mouse
• species reactivity (predicted by homology): human
• packaging: antibody small pack of 25 μL
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Sustainability features
• technique(s): immunofluorescence: suitable using 1:100, immunohistochemistry: suitable using 1:100, western blot: suitable using 1:1,000
• isotype: IgG
• UniProt accession no.: P28329
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... CHAT(1103)

Opis

General description

Choline O-acetyltransferase (UniProt: P28329; also known as EC: 2.3.1.6, CHOACTase, ChAT, Choline acetylase) is encoded by the CHAT gene (Gene ID: 1103) in human. Choline acetyltransferase a single-strand globular protein that catalyzes the biosynthesis of acetylcholine from acetyl CoA and choline. It is one of the most specific indicator for monitoring the functional state of cholinergic neurons in the central and peripheral nervous systems. It is synthesized in the perikaryon of cholinergic neurons and transported to the nerve terminals. Mutations in CHAT gene are known to cause Myasthenic syndrome that is characterized by failure of neuromuscular transmission, which include pre-synaptic, synaptic, and post-synaptic disorders. Three isoforms of Choline acetyltransferase have been described that are produced by alternative splicing. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Oda, Y et al., (1999). Pathol. Int. 49(11); 921-937).

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

This product aligns with one or more of the 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Immunogen

KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal half of human Choline acetyltransferase.

Application

Anti-Choline Acetyltransferase (ChAT), clone 2K16 ZooMAb, Cat. No. ZRB1012, is a recombinant Rabbit Monoclonal Antibody that specifically targets Choline Acetyltransferase (aka ChAT) and has been tested for use in Immunofluorescence, Immunohistochemistry, and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Choline Acetyltransferase (ChAT) in mouse brain tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Choline Acetyltransferase (ChAT) in rat brain tissue sections.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Choline Acetyltransferase (ChAT) in mouse brain tissue sections.

Biochem/physiol Actions

Clone 2K16 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Choline Acetyltransferase. It targets an epitope with in 19 amino acids from the N-terminal half.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Preparation Note

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• Co się dzieje?: WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable