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Merck

Novel substrates of yeast alcohol dehydrogenase-3. 4-dimethylamino-cinnamaldehyde and chloroacetaldehyde.

Biochemistry and molecular biology international (1997-11-14)
V Leskovac, S Trivić, J Zeremski, B Stancić, B M Anderson
ABSTRAKT

4-Dimethylamino-trans-cinnamaldehyde and chloroacetaldehyde are novel substrates of yeast alcohol dehydrogenase (EC 1.1.1.1). In the present work, we have reported the steady-state kinetic constants for both substrates, and their chemical reactions with the enzyme protein itself. Both substrates are potentially useful for biotechnology, chemoenzyme syntheses and analytical biochemistry.

MATERIAŁY
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Millipore
DMACA Reagent, suitable for microbiology
Sigma-Aldrich
4-(Dimethylamino)cinnamaldehyde, chromogenic reagent for indoles and flavanols