[Protein determination by binding with the dye Coomassie brilliant blue G-250].

Possible applications of the Coomassie brilliant blue G-250 dye method for protein assay are reviewed. The physico-chemical and optical properties of the dye are considered in detail. The data on the protein-binding mechanism are generalized. Modifications of the Coomassie blue G-250 quantitative protein assays are critically considered. The applications of the method in medicine, biochemistry and biotechnology are discussed.