powder or crystals
(1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)
252.6 °C (lit.)
water: 100 mg/mL, clear, colorless
2.338 g/mL at 25 °C (lit.)
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This procedure applies to all products that have a specification for Cathepsin B activity determined by the liberation of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin.
Proteinase K (EC 188.8.131.52) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol of tyrosine per minute at pH 7.5 at 37 °C (color by Folin & Ciocalteu's Phenol Reagent).
Derived from procedure SSPHYT02. Includes template updates to current SOP specifications and incorporation of notes into the procedure.
This procedure may be used for all Alcohol Oxidase products. The continuous spectrophotometric rate determination (A405, Light path = 1 cm) is based on the following reactions.
This is a protocol to be used for all Malic Dehydrogenase products.
This procedure applies to products that have a specification for the enzymatic activity of cholesterol oxidase. This assay is NOT to be used to assay Cholesterol Oxidase from Schizophyllum commune (Discontinued Product Number C7274) and from Brevibacterium sp. (Discontinued Product Number C8153).